Protein Info for SMc01105 in Sinorhizobium meliloti 1021

Updated annotation (from data): uridine nucleosidase (EC 3.2.2.3)
Rationale: Specifically important for: Uridine. annotated by SEED as acting on uridine and inosine
Original annotation: nucleoside hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 7 to 305 (299 residues), 365.1 bits, E=1.9e-113

Best Hits

Swiss-Prot: 54% identical to YHD6_SCHPO: Uncharacterized protein C1683.06c (SPBC1683.06c) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01239, purine nucleosidase [EC: 3.2.2.1] (inferred from 100% identity to sme:SMc01105)

MetaCyc: 51% identical to pyrimidine-specific ribonucleoside hydrolase RihA (Escherichia coli K-12 substr. MG1655)
Uridine nucleosidase. [EC: 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.3]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of pyrimidine ribonucleosides salvage (8/10 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• NAD salvage (plants) (7/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.1

Use Curated BLAST to search for 3.2.2.1 or 3.2.2.3

Sequence Analysis Tools

See Q92SI0 at UniProt or InterPro

Protein Sequence (314 amino acids)

>SMc01105 uridine nucleosidase (EC 3.2.2.3) (Sinorhizobium meliloti 1021)
MSSARKIIIDTDPGQDDAAAIMLALGSPEEIEVLGITAVAGNVPLTLTARNARIVCELCN
RTDVKIFAGAERPVARPLVTAEHVHGKTGLDGPALDEPTMPLQEQHAVDFIVETLRAEAP
GAVTLCTLGPLTNIALALTKAPEIAPSVRELVMMGGGFFEGGNITPAAEFNIYVDPEAAE
IVFRSGIPIVMMPLDVTHRVLTRKTRVEKIRAIGSPAAVALAEMLEFFERFDIEKYGTDG
GPLHDPTVIAYILRPELFTGRDCNVEIETASPLTTGMTVVDWWQVTGRAHNAKVMRHIDD
EGFFELLAERLARI