Protein Info for SMc01053 in Sinorhizobium meliloti 1021

Annotation: siroheme synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR01470: siroheme synthase, N-terminal domain" amino acids 8 to 209 (202 residues), 202.1 bits, see alignment E=7.9e-64 PF13241: NAD_binding_7" amino acids 11 to 119 (109 residues), 72.1 bits, see alignment E=7.8e-24 PF10414: CysG_dimeriser" amino acids 154 to 211 (58 residues), 56.7 bits, see alignment 2.4e-19 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 219 to 453 (235 residues), 291.9 bits, see alignment E=4.3e-91 PF00590: TP_methylase" amino acids 221 to 430 (210 residues), 179.9 bits, see alignment E=1e-56

Best Hits

Swiss-Prot: 41% identical to CYSG_METCA: Siroheme synthase (cysG) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 100% identity to sme:SMc01053)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92Q80 at UniProt or InterPro

Protein Sequence (485 amino acids)

>SMc01053 siroheme synthase (Sinorhizobium meliloti 1021)
MSQKLSVFPAFFRVAQKSVAVFGNGDEAFAKLRLLLNTEARIVAYADRPEPAFDAFLDAN
NIETVREPFGAHQVEGSVLVFAATGDAALDRVVVTAARERKIPANAVDQPDYCDFLTPAL
VNRAPVAVAIGTEGAGPVLAQMIRAQIDQLLSPSLGIVASLAANYRDAVERLVPRGVARR
VFWRRFFSGPVADQVALGDLASARREASRLLDAAGGVAGHVWLVGAGPGAEDLLTLRAQR
VMMEADAIVYDALVPQAIVDMGRRDAERLSVGKRKGCHTKSQDEINQLLVKLAGEGKRVV
RLKSGDPLVYGRAGEEMAALREAGISYEVVPGITSAFAAAADFELPLTLRGVASSLVFTT
GHDLTGSVLPDWARLAVSGATIAVYMGRTVAASVASRLMQAGLPQDTTVAVIENASRAER
RLLHGTLRDLPDLEARTELDGPVMVIIGEAVAGANFERSEPLAAGRTKYSGASKVSAKRT
EQIWN