Protein Info for SMc01044 in Sinorhizobium meliloti 1021

Annotation: nitrogen regulation transmembrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 753 transmembrane" amino acids 23 to 49 (27 residues), see Phobius details amino acids 55 to 75 (21 residues), see Phobius details amino acids 96 to 119 (24 residues), see Phobius details amino acids 294 to 319 (26 residues), see Phobius details PF19312: NtrY_N" amino acids 68 to 301 (234 residues), 135.5 bits, see alignment E=5.1e-43 PF00672: HAMP" amino acids 318 to 369 (52 residues), 42.7 bits, see alignment 1.2e-14 PF00512: HisKA" amino acids 506 to 572 (67 residues), 41 bits, see alignment E=3.3e-14 PF02518: HATPase_c" amino acids 616 to 734 (119 residues), 71 bits, see alignment E=2.3e-23

Best Hits

KEGG orthology group: K13598, two-component system, NtrC family, nitrogen regulation sensor histidine kinase NtrY [EC: 2.7.13.3] (inferred from 100% identity to sme:SMc01044)

Predicted SEED Role

"Nitrogen regulation protein NtrY (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92Q88 at UniProt or InterPro

Protein Sequence (753 amino acids)

>SMc01044 nitrogen regulation transmembrane protein (Sinorhizobium meliloti 1021)
MALPLGTEDGVTAAQDRRASFALPGLMLATGALICATLSLLVLLGLTPIRPERNIVIACA
GINGLFVVGLIYLIAREIFRLLRARSKGRAAARLHVRIVALFSIVAITPAILVAIFASIT
LDVGLDRWFSLRTQAIVRSSLNVAQAYVLENASYLQGQTVSMANDLERNRQLYSLDRTGF
VELMTRQARGRGMLGAFLVRADGSAILQANISTDRPLPAIPQDALKSTIAGQPTLIPPGV
TNLVGAVIPLENIPGTYLYTVRNVDPEVMRSMRLMEENTAEYKTLEAGRTSLQIAFGVLY
IGFALIVLLAAIWTAIAVADRIVRPIRQLIGAADSVASGNLDVVVPVRAVDGDVGNLSRT
FNKMVSEIRTQQDQILVAKDEVDQRRRFIEAVLSGVTAAVIGVGRDRRITIFNPSSEGML
KKAAPELIGANLSDVAPEIEAVLVEAESRYRNDYRKQINIMRGGTERTMNVQVTREEGDE
SHGSYVITVDDITDLVIAQRSTAWADVARRIAHEIKNPLTPIQLSAERLKRRYGKQIDQE
DRAVFDQCTETIVRQVEDIGRMVDEFSAFARMPKPTKEKSDLRAILKDAVFLREMGNSHI
NFVRDFGDEPLEGQFDGRMLGQAFGNLVKNAVEAIEAVPAGTSRGAPTVVIRSRRDDTTG
RFVVDVIDNGKGLPTENRHRILEPYMTMRDKGTGLGLAIVKKIIEDHGGQLELHDAPPDF
DGGAGAMIRVVLPPAGETGGEDNLKDKGNTNGG