Protein Info for SMc00909 in Sinorhizobium meliloti 1021

Annotation: bifunctional riboflavin kinase/FMN adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 33% identical to RIBF_BUCAI: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to sme:SMc00909)

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
flavin biosynthesis I (bacteria and plants) (8/9 steps found)
5,6-dimethylbenzimidazole biosynthesis I (aerobic) (3/3 steps found)
flavin salvage (2/2 steps found)
flavin biosynthesis III (fungi) (7/9 steps found)
flavin biosynthesis II (archaea) (5/10 steps found)
roseoflavin biosynthesis (1/7 steps found)

KEGG Metabolic Maps

Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92RR1 at UniProt or InterPro

Protein Sequence (327 amino acids)

>SMc00909 bifunctional riboflavin kinase/FMN adenylyltransferase (Sinorhizobium meliloti 1021)
MTVFHRNETRDPLPEHLRGGVVAIGNFDGVHRGHQSVLNRALEEAVKRAVPALVLTFEPH
PRTVFRPDTPVFRLTPAPLKARILEGMGFGAVIEYPFDRSFSELSASDFIHSILREWLHA
SHVVTGFDFHFGKGREGGPAFLMAAGEREGFGVTLVDAFRDENAAVISSSFIRSLLADGD
VPHAAGLLGYRYTVEAEVIDGKKLGRTLGYPTANMRLPPEAELKSGIYAVRFRRADGSLH
DGVASFGRRPTVDSDGEALLETFVFDFSADLYGEICAVSFFGRLRDELKFDGLEPLMVQM
RKDESEARALLAGVQPLSGIDRRLNFA