Protein Info for SMc00884 in Sinorhizobium meliloti 1021
Annotation: dihydroxy-acid dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ILVD1_RHIME: Putative dehydratase IlvD1 (ilvD1) from Rhizobium meliloti (strain 1021)
KEGG orthology group: K01687, dihydroxy-acid dehydratase [EC: 4.2.1.9] (inferred from 100% identity to smk:Sinme_0529)Predicted SEED Role
"Galactonate dehydratase (EC 4.2.1.6)" in subsystem D-galactonate catabolism (EC 4.2.1.6)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-valine biosynthesis (4/4 steps found)
- superpathway of L-threonine metabolism (14/18 steps found)
- D-galactonate degradation (3/3 steps found)
- pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- L-isoleucine biosynthesis II (5/8 steps found)
- L-isoleucine biosynthesis III (4/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Galactose metabolism
- Pantothenate and CoA biosynthesis
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.6, 4.2.1.9
Use Curated BLAST to search for 4.2.1.6 or 4.2.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q92RP0 at UniProt or InterPro
Protein Sequence (574 amino acids)
>SMc00884 dihydroxy-acid dehydratase (Sinorhizobium meliloti 1021) MSDKKKELRSRHWYGGTHKDGFIHRSWMKNQGFPDHVFDGRPIIGICNTWSELTPCNSHL RILAEGVKRGVWEAGGFPVEFPVSSLGETQMRPTAMLFRNLLAMDVEEAIRAYGIDGVVL LGGCDKTTPGQLMGAASVDLPTIVVSSGPMLNGKWKGKDIGSGTDVWKFSEAVRAGEMSL QEFMAAESGMSRSPGVCMTMGTATTMASIVEAMGLSLPTNAALPAVDARRMALSHMTGKR IVEMVHEDLRLSKILTKENFENGIIANAAVGGSTNAVVHMLAIAGRAGIDLCLEDFDRVG GQVPCIVNCMPSGKYLIEDLAYAGGLPAVMNRIQHLLHPDAPTVFGVPISKYWEAAEVYN DDVIRPLDNPLRAAAGIRVLKGNLAPNGAVIKPSAASEHLLAHEGPAYVFDTIEDLRAKI DDPDLPVTEDTILVLKGCGPKGYPGMAEVGNMPIPRRLVEKGVRDMVRISDARMSGTAFG TVVLHVSPEANAGGPLAIVRTGDRIRLDALKGELNLLVSEEELAARMAAWQPPEQKWHRG YYKLYHDTVLQADKGADLDFLVGKSGSEVLRESH