Protein Info for SMc00701 in Sinorhizobium meliloti 1021

Annotation: cobalamin biosynthesis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 PF06213: CobT" amino acids 14 to 285 (272 residues), 324.6 bits, see alignment E=6e-101 TIGR01651: cobaltochelatase, CobT subunit" amino acids 17 to 618 (602 residues), 1166.2 bits, see alignment E=0 PF11775: CobT_C" amino acids 399 to 616 (218 residues), 372.2 bits, see alignment E=9.2e-116

Best Hits

Swiss-Prot: 91% identical to COBT_SINSX: Aerobic cobaltochelatase subunit CobT (cobT) from Sinorhizobium sp.

KEGG orthology group: K09883, cobaltochelatase CobT [EC: 6.6.1.2] (inferred from 100% identity to sme:SMc00701)

MetaCyc: 91% identical to CobT (Pseudomonas denitrificans (nom. rej.))

Predicted SEED Role

"Aerobic cobaltochelatase CobT subunit (EC 6.6.1.2)" in subsystem Coenzyme B12 biosynthesis or Conenzyme B12 related Hypothetical: Clusters with cobST (EC 6.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.6.1.2

Use Curated BLAST to search for 6.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92MF2 at UniProt or InterPro

Protein Sequence (631 amino acids)

>SMc00701 cobalamin biosynthesis protein (Sinorhizobium meliloti 1021)
MSSNSKAKPTTRENAAEPFKRALSGCIRSIAGDAELEVAFANERPGMTAERIRLPELSKR
PTRQELAVARGLGDSMALRKACHDARVHATMSPQGADARAIFDAVEQARVEAIGALRMPG
VAGNLSSMLEEKYSKANFAAIDAQSDAPLEEAVALLVREKLTGEKPPESAGQVLDLWRDF
IEQKAGPDMRNLAGTINDQQAFARVVRDMLSSMDVAEKYGEDDNEPDEQESETDEDQPRS
QEQDENASDEEEGSDAAPADENQSAEEQMEEGEMDGAEISDDDLQDESDEDSETPGEVKR
PNHPFADFNEKVDYSVYTREFDETIASEELCDEAELDRLRAFLDKQLAHLQGAVGRLANR
LQRRLMAQQNRSWEFDLEEGYLDSARLQRIIIDPMQPLSFKREKDTNFRDTVVTLLIDNS
GSMRGRPITVAATCADILARTLERCGVKVEILGFTTKAWKGGQSREKWLAGGKPQSPGRL
NDLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALMWAHDRLLARSEQRRILMMI
SDGAPVDDSTLSVNPGNYLERHLRAVIEQIETRSPVELLAIGIGHDVTRYYRRAVTIVDA
DELAGAMTDQLAALFEDESMRRRPGGARRAG