Protein Info for SMc00618 in Sinorhizobium meliloti 1021

Annotation: polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 TIGR03705: polyphosphate kinase 1" amino acids 34 to 709 (676 residues), 940 bits, see alignment E=4.1e-287 PF13089: PP_kinase_N" amino acids 35 to 139 (105 residues), 120.4 bits, see alignment E=1.1e-38 PF02503: PP_kinase" amino acids 149 to 326 (178 residues), 193.7 bits, see alignment E=5.6e-61 PF17941: PP_kinase_C_1" amino acids 354 to 518 (165 residues), 251.2 bits, see alignment E=9.7e-79 PF13090: PP_kinase_C" amino acids 525 to 703 (179 residues), 201.8 bits, see alignment E=1.5e-63

Best Hits

Swiss-Prot: 66% identical to PPK1_AZOC5: Polyphosphate kinase (ppk) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to smk:Sinme_0983)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QV9 at UniProt or InterPro

Protein Sequence (736 amino acids)

>SMc00618 polyphosphate kinase (Sinorhizobium meliloti 1021)
MDSATSAITEHEASAEQKREAPAEALDLLKSPERFVNREFSWLQFNRRVLEETLNTAHPL
LERVRFLSISAANLDEFFMVRVAGLEGQVRSGLSLRSPDGLTPAETLESILTEVDNLQME
QQASLAVLQQYLAKEDILIVRPASLSAQDRSWLANEFDQAIFPVLTPLSIDPAHPFPFIP
NLGFSIGLQLVSKTGRDPMTALLRLPVALDRFVRLPDVKNVIRYITLEDVVSQFIDRLFP
GYEVKGSGTFRIIRDSDIEVEEEAEDLVRLFETALKRRRRGSVIRIETDSEMPASLRQFV
VQELGVPENRVAVLPGLLALNTLSEITKAPREDLRFEPYNPRFPERVREHAGDCLAAIRE
KDMVVHHPYESFDVVVQFLLQAARDPDVLAIKQTLYRTSNDSPIVRALIDAAEAGKSVTA
LVELKARFDEEANIRWARDLERAGVQVVFGFIELKTHAKMSMVVRREEGKLRTYCHLGTG
NYHPVTAKIYTDLSFFTCNPVIAHDMANIFNFITGYGEPEGGMKLAVSPHTLRPRILKHI
EEEIVHAQAGRPASIWMKMNSLVDPEIIDALYKASCAGVVIDLVVRGICCLRPQVPGLSD
NIRVKSIVGRFLEHSRIFCFGNGHGLPSENALVYIGSADMMPRNLDRRVETLVPLINRTV
HEQVLSQIMLGNLIDNQQSYEILPDGTSRRMEVGKDAEPFNAQHYFMTNPSLSGRGEALK
SSAPKLIAGWKSGWHK