Protein Info for SMc00588 in Sinorhizobium meliloti 1021

Updated annotation (from data): L-arabinose 1-dehydrogenase; D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48)
Rationale: Important on L-arabinose and D-galactose; may also act on D-glucose, given phenotype with some N sources with glucose as C source; 55% identical to ARAA_AZOBR, which has L-arabinose and D-galactose 1-dehydrogenase activity in vitro
Original annotation: D-galactose 1-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 PF01408: GFO_IDH_MocA" amino acids 4 to 114 (111 residues), 66.6 bits, see alignment E=1.7e-22

Best Hits

Swiss-Prot: 55% identical to ARAA_AZOBR: L-arabinose 1-dehydrogenase (NAD(P)(+)) (araA) from Azospirillum brasilense

KEGG orthology group: K00035, D-galactose 1-dehydrogenase [EC: 1.1.1.48] (inferred from 100% identity to smk:Sinme_0954)

MetaCyc: 100% identical to galactose dehydrogenase (Sinorhizobium meliloti)
Galactose 1-dehydrogenase. [EC: 1.1.1.48]

Predicted SEED Role

"Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-)" (EC 1.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.1.1.46 or 1.1.1.48

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92QY5 at UniProt or InterPro

Protein Sequence (308 amino acids)

>SMc00588 L-arabinose 1-dehydrogenase; D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (Sinorhizobium meliloti 1021)
MSPINIAIVGVGKIVRDQHLPALAKNADYRLIAAASRHGTVDGIDNFKSIEAMIDAVPAV
EAVSLCMPPQYRYEAARTALAAGKHVFLEKPPGATLSEVADLEALAEEKGVSLFASWHSR
YAPAVEAAKTFLASAAIRNVRIIWKEDVRHWHPNQEWIWAAGGLGVFDPGINALSIMTHI
LPRPVFITSATLEFPENRDAPIAATIAFSDAEKLDVAAEFDWRQTGKQSWDIVAETDAGG
MVLSEGGAKLAIDGKIVHEEPEQEYPMLYRRFAEIIKAGRSDVDLAPLRHVADAFMLGRR
KFVEAFHD