Protein Info for SMc00475 in Sinorhizobium meliloti 1021

Annotation: alanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 6 to 555 (550 residues), 732.8 bits, E=4e-224
• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 868 (863 residues), 962.3 bits, E=1.3e-293
• PF07973: tRNA_SAD
  • amino acids 653 to 706 (54 residues), 54.3 bits, 1.6e-18
• PF02272: DHHA1
  • amino acids 742 to 882 (141 residues), 78.2 bits, E=1.3e-25

Best Hits

Swiss-Prot: 80% identical to SYA_AGRFC: Alanine--tRNA ligase (alaS) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 85% identity to ara:Arad_2617)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• superpathway of chorismate metabolism (38/59 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See P27866 at UniProt or InterPro

Protein Sequence (887 amino acids)

>SMc00475 alanyl-tRNA synthetase (Sinorhizobium meliloti 1021)
MSGVNEIRSMFLDYFRKNGHEIVSSSPLVPRNDPTLMFTNAGMVQFKNVFTGLEQRPYST
AATAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKERAIELAWNLITKEYGL
DAKRLLVTVYHTDDEAFGLWKKIAGLSDDRIIRIATSDNFWAMGDTGPCGPCSEIFYDHG
DHIWGGPPGSAEEDGDRFIEIWNLVFMQYEQITKEERVDLPRPSIDTGMGLERVAAVLQG
QHDNYDIDLFRALIAASEEATGVKAEGDRRASHRVIADHLRSSAFLIADGVLPSNEGRGY
VLRRIMRRAMRHAQLLGARDPLMWKLLPALVGQMGRAYPELVRAEALISETLKLEETRFR
KTLERGLNLLAEASADLSEGDQFNGETAFKLYDTYGFPLDLTQDALRAKGIGVDTDAFTA
AMQRQKAEARANWAGSGEAATETIWFELRDKHGATDFLGYDTESAEGVILAIVKDGAVVE
SAAKGENVQLVLNQTPFYGESGGQVGDTGVITTETGKLTVTDTQKRGEGLFVHYCTVEEG
SVKTGEAAALTVDHARRARLRANHSATHLLHEALREVLGTHVAQKGSLVAPERLRFDVSH
PKPMTAEELKIVEEMANEIIVQNTPVVTRLMSVDDAIAEGAMALFGEKYGDEVRVVSMGQ
GLRGSKAGKPYSVELCGGTHVSATGDIGLVRVVSESAVGAGVRRVEALTGEAARAYLGEQ
DERVKTLAAALKVQPADVLGRVEALLDERRKLERELTEAKKKLALAGDGQNGSGDAARDI
GGVRFLGRVVSGVEPKDLKSLADDGKKSLGSGVVAFVGVSGDGKASAVVAVTDDLTSKVS
AVDLVRVASAALGGKGGGGRPDMAQAGGPDGGRAAEAIEAVAGALAG