Protein Info for SMc00349 in Sinorhizobium meliloti 1021

Annotation: GTP-binding protein LepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 12 to 190 (179 residues), 184.9 bits, E=2.9e-58
• TIGR01393: elongation factor 4
  • amino acids 12 to 605 (594 residues), 974.2 bits, E=2.2e-297
• TIGR00231: small GTP-binding protein domain
  • amino acids 15 to 180 (166 residues), 65.7 bits, E=4.3e-22
• PF01926: MMR_HSR1
  • amino acids 23 to 141 (119 residues), 22.9 bits, E=1.9e-08
• PF03144: GTP_EFTU_D2
  • amino acids 216 to 286 (71 residues), 47.6 bits, E=4.5e-16
• PF00679: EFG_C
  • amino acids 410 to 494 (85 residues), 67.6 bits, E=2e-22
• PF06421: LepA_C
  • amino acids 498 to 604 (107 residues), 170.6 bits, E=2.3e-54

Best Hits

Swiss-Prot: 100% identical to LEPA_RHIME: Elongation factor 4 (lepA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to smk:Sinme_3599)

MetaCyc: 58% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See Q92SU3 at UniProt or InterPro

Protein Sequence (608 amino acids)

>SMc00349 GTP-binding protein LepA (Sinorhizobium meliloti 1021)
MNTPSSKTPLSHIRNFSIVAHIDHGKSTLADRLIQSTGGLAEREMSEQVLDSMDIERERG
ITIKAQTVRLHYKANDGETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQ
TLANVYQAIDNNHELVTVLNKIDLPAAEPERIKEQIEEVIGIDASDAVLISAKTGLGIPD
VLEAIVHKLPAPKSEGGDTAPLKALLVDSWYDAYLGVMVLVRVIDGTLKKGMTIRMMGTD
AKYQVERVGVLTPKMVAMEALGPGEIGFITASIKEVADTRVGDTITEDKRPTAKALPGFK
PAQPVVFCGLFPVDAADFEDLRSAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLE
IIQERLEREFDLDLIATAPSVVYKLFMTDGSERELHNPADMPDVVKIAEIHEPWIRATIL
TPDEYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYASFD
YQITEHKEGNLVKMSILVNGEPVDALSMMVHRMAAEKRGREMCEKLKELIPKHMFKIPIQ
AAIGGNVIARETISALRKDVTAKCYGGDATRKRKLLEKQKAGKKRMRQFGKVEIPQEAFI
AALKMSDE