Protein Info for SMc00232 in Sinorhizobium meliloti 1021

Annotation: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 6 to 117 (112 residues), 31.5 bits, E=4.6e-11
• PF00483: NTP_transferase
  • amino acids 7 to 149 (143 residues), 50 bits, E=9.1e-17
• PF02348: CTP_transf_3
  • amino acids 7 to 137 (131 residues), 28.4 bits, E=4.3e-10
• PF12804: NTP_transf_3
  • amino acids 7 to 141 (135 residues), 77.8 bits, E=3.4e-25
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 432 (426 residues), 466 bits, E=6.8e-144
• PF14602: Hexapep_2
  • amino acids 380 to 413 (34 residues), 37.9 bits, 3.6e-13
• PF00132: Hexapep
  • amino acids 380 to 413 (34 residues), 32.2 bits, 1.9e-11

Best Hits

Swiss-Prot: 100% identical to GLMU_RHIME: Bifunctional protein GlmU (glmU) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to sme:SMc00232)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See Q92PS3 at UniProt or InterPro

Protein Sequence (456 amino acids)

>SMc00232 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (Sinorhizobium meliloti 1021)
MERTCLAIILAAGESTRMKSAMSKVLHPVAGRPMIAHVVDALASASISDVALVVGRDADA
VSAAAATDEVAVTSFLQKERLGTAHAVLAAREAIAKGYDDVLVVFGDTPLITAAPLKAAR
DGLAAGNDVVVIGFQATDPTGYGRLIVKDGALVAIREHRDASDEERRITYCNGGLMAIDG
RKALDLLNRIGNANAKGEYYLTDLVEIARSLDGRAIAVEAPEEELTGCNTRAELAYIERL
WQQRRRHELMLAGVSMIAPETVFLSWDTALAQDVLLEPNVVFGPGVRVESGAVIHAFSHL
EGAHVRAGATVGPFARLRPGADLGAKSKVGNFCEVKNAEIGAGAKVNHLTYIGDAFVGAG
SNIGAGTITCNYDGVNKHVTRIGANAFVGSNSSLVAPVSIGDGALVASGSVITEDVPADA
VAFGRARQDVKPGRAPILRERYEAEKAARKRAKAAE