Protein Info for SMc00092 in Sinorhizobium meliloti 1021

Annotation: phosphoadenosine phosphosulfate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01507: PAPS_reduct
  • amino acids 50 to 224 (175 residues), 128.9 bits, E=1.1e-41
• TIGR02055: adenylylsulfate reductase, thioredoxin dependent
  • amino acids 55 to 248 (194 residues), 286 bits, E=7.6e-90

Best Hits

Swiss-Prot: 100% identical to CYSH_RHIME: Phosphoadenosine phosphosulfate reductase (cysH) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00390, phosphoadenosine phosphosulfate reductase [EC: 1.8.4.8] (inferred from 100% identity to sme:SMc00092)

MetaCyc: 100% identical to phosphoadenylyl-sulfate reductase (thioredoxin) (Sinorhizobium meliloti)
Adenylyl-sulfate reductase (thioredoxin). [EC: 1.8.4.10]

Predicted SEED Role

"Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10)" in subsystem Cysteine Biosynthesis (EC 1.8.4.10, EC 1.8.4.8)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.4.10 or 1.8.4.8

Sequence Analysis Tools

See P56891 at UniProt or InterPro

Protein Sequence (265 amino acids)

>SMc00092 phosphoadenosine phosphosulfate reductase (Sinorhizobium meliloti 1021)
MTTQSLKAEAVALEADVMALDAEAKALNDKLESLDLAGRLALIAGLEGRAVFTTSLGIED
QVITAAIGSNRLDIEVATLKTGRLFNETVALIDQTEETYDILIKRYYPEKADIDAYVAQY
GMNGFYESVEARHACCGVRKLKPLARALDGASYWITGLRRGQSGNRATTPFAEADVERGL
IKINPLADWGIETIQAHVAAEGIPVNPLHSRGYPSIGCEPCTRAIKPGEPERAGRWWWEN
DEKRECGLHVPEAASSIIPNASNAA