Protein Info for SMc00069 in Sinorhizobium meliloti 1021

Annotation: pyridoxamine 5'-phosphate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 206 TIGR00558: pyridoxamine 5'-phosphate oxidase" amino acids 15 to 206 (192 residues), 234 bits, see alignment E=7.2e-74 PF01243: Putative_PNPOx" amino acids 25 to 111 (87 residues), 98.2 bits, see alignment E=3.9e-32 PF12766: Pyridox_oxase_2" amino acids 27 to 107 (81 residues), 37.9 bits, see alignment E=3.4e-13 PF10590: PNP_phzG_C" amino acids 164 to 206 (43 residues), 63.3 bits, see alignment 2.3e-21

Best Hits

Swiss-Prot: 100% identical to PDXH_RHIME: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 100% identity to smk:Sinme_0602)

MetaCyc: 43% identical to pyridoxine phosphate oxidase (Saccharomyces cerevisiae)
Pyridoxal 5'-phosphate synthase. [EC: 1.4.3.5]; 1.4.3.5 [EC: 1.4.3.5]

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92RH8 at UniProt or InterPro

Protein Sequence (206 amino acids)

>SMc00069 pyridoxamine 5'-phosphate oxidase (Sinorhizobium meliloti 1021)
MTANELITGDFTDASEPFSLFGTWLKEAEKNEVNDPNAVALATVDPDGMPNVRMVLLKGF
DERGFVLYTNFESQKGREMLATRKAALCFHWKSLRRQVRLRGPVEIVSDEEADEYFMSRP
RGSRIGAWASKQSRPLESRFALEKAVAEYTARHAIGEIPRPDYWSGFRIRPLSIEFWHDR
PFRLHDRVEFRRETPDGGWTKVRMYP