Protein Info for SMa2143 in Sinorhizobium meliloti 1021

Annotation: 5,10-methylenetetrahydrofolate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02219: MTHFR
  • amino acids 91 to 245 (155 residues), 25 bits, E=6e-10

Best Hits

KEGG orthology group: K00297, methylenetetrahydrofolate reductase (NADPH) [EC: 1.5.1.20] (inferred from 100% identity to sme:SMa2143)

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

• folate transformations I (9/13 steps found)

KEGG Metabolic Maps

• Methane metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.20

Use Curated BLAST to search for 1.5.1.20

Sequence Analysis Tools

See Q92XS4 at UniProt or InterPro

Protein Sequence (317 amino acids)

>SMa2143 5,10-methylenetetrahydrofolate reductase (Sinorhizobium meliloti 1021)
MVAALAAANGICLFENPGEAMTNPAEACTDTRVHPVDGADTTISIELAPEQVRNFAPQAD
TLALGSRVFLTHLAGKPEVLQVEAAARIIEMGYVAVPHLAARNFKSERDYISHVEAHSRN
GIDEVLFLGGNPALFPGPLGESAELLAHPVLSDSSIRTAFVAGYPEGHPNISEARLKDAL
KRKLEICARRSLEPRIVSQFAFDGAMIGAWAKRLHDDFPEVPIHVGLAGVTSLTKLIRFA
AMCGVGPSIAALRRSVSGLLNIVADRNPADVIDAVSKAYPDTVTPLHLHFFPFGGWEKTL
TWLGDYRVARQLQAGVR