Protein Info for SMa1872 in Sinorhizobium meliloti 1021
Annotation: threonine dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 38% identical to SRR_DICDI: Probable serine racemase (srr) from Dictyostelium discoideum
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 100% identity to sme:SMa1872)MetaCyc: 36% identical to Serine racemase (Homo sapiens)
Amino-acid racemase. [EC: 5.1.1.10, 5.1.1.18]
Predicted SEED Role
"Threonine dehydratase, catabolic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Glycine and Serine Utilization or Threonine anaerobic catabolism gene cluster or Threonine degradation (EC 4.3.1.19)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- superpathway of L-threonine metabolism (14/18 steps found)
- L-methionine degradation II (3/3 steps found)
- L-threonine degradation I (5/6 steps found)
- alanine racemization (1/1 steps found)
- serine racemization (1/1 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- L-alanine degradation I (1/2 steps found)
- L-threonine degradation V (1/2 steps found)
- vancomycin resistance II (1/3 steps found)
- L-lysine degradation V (5/9 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (2/6 steps found)
- ansatrienin biosynthesis (1/9 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
- superpathway of L-lysine degradation (15/43 steps found)
KEGG Metabolic Maps
- Cysteine metabolism
- D-Arginine and D-ornithine metabolism
- D-Glutamine and D-glutamate metabolism
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.19
Use Curated BLAST to search for 4.3.1.19 or 5.1.1.10 or 5.1.1.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q92Y58 at UniProt or InterPro
Protein Sequence (323 amino acids)
>SMa1872 threonine dehydratase (Sinorhizobium meliloti 1021) MRNYPTIQDIREARERLKPHVRHTPLLRAEKIEKAAGCQLYLKPETLQITGAFKIRGALN KALSLSREEIANGIIATSSGNHAQGLSYAAKMLGVKVILVLPVTTPKIKIENTKALGAEV ILFDGDNAARWKKVYEIAEGNKYAVIHGFEDPVVMAGQGTIGCEILEDLDDVDTVIVPLG GGGLISGIATAIKETKPSVRVIGAEPALTPKYFHSRVNKERTSLPLKNTIADGLRISVPG QNPYPIIEKYVDEIVLVEDEHIIAGMRALAKDAKLIAEPAASIGVGALLAGIIDVKLDEK VCAVLSGGNWDLRDLAAIYNVAG