Protein Info for SMa1583 in Sinorhizobium meliloti 1021

Annotation: CyaF5 adenylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 589 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00211: Guanylate_cyc" amino acids 14 to 165 (152 residues), 38.9 bits, see alignment E=4.9e-13 PF07719: TPR_2" amino acids 424 to 455 (32 residues), 24.4 bits, see alignment (E = 1.5e-08) PF13181: TPR_8" amino acids 426 to 455 (30 residues), 14.6 bits, see alignment (E = 2.2e-05) amino acids 457 to 489 (33 residues), 15.8 bits, see alignment (E = 9.1e-06) PF13424: TPR_12" amino acids 427 to 487 (61 residues), 33.7 bits, see alignment E=2.4e-11 PF13432: TPR_16" amino acids 427 to 489 (63 residues), 27.4 bits, see alignment E=2.6e-09 amino acids 497 to 549 (53 residues), 19.2 bits, see alignment 9.5e-07 PF13431: TPR_17" amino acids 446 to 477 (32 residues), 27.5 bits, see alignment (E = 1.7e-09) PF13176: TPR_7" amino acids 459 to 489 (31 residues), 17 bits, see alignment (E = 3.4e-06) PF14559: TPR_19" amino acids 470 to 523 (54 residues), 25.9 bits, see alignment 7.1e-09

Best Hits

Swiss-Prot: 100% identical to CYA3_RHIME: Putative adenylate cyclase 3 (cya3) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01768, adenylate cyclase [EC: 4.6.1.1] (inferred from 100% identity to sme:SMa1583)

Predicted SEED Role

"Adenylate cyclase (EC 4.6.1.1)" in subsystem cAMP signaling in bacteria (EC 4.6.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.6.1.1

Use Curated BLAST to search for 4.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q9Z3Q0 at UniProt or InterPro

Protein Sequence (589 amino acids)

>SMa1583 CyaF5 adenylate cyclase (Sinorhizobium meliloti 1021)
MRMAKESIRRRLAAILAADAVGYSRLMERDEKSTHTLLMARWKEVLEPLVGIHQGRVFKR
TGDGVLVEFGSAVNAVECAAALQQAMAAANRDLPEDRAIVLRVGVNLGDIMVEDSDLFGD
GVNVAARIEALADPGGVAISDGIHEYVHGRTDIDFVDSGYHEVKNIERPVHIWTWSPKDR
AREPPNIAAEPPPQLPAKPSIAVLPFDNMSGDPEQGYFADGITEDIITDLSKVSGLFVIA
RNSSFAYKGKTPDIRKVSRELGVRYVLEGSVRRAANRIRINAQMIDGTTGGHLWAERYDR
GLEDIFAVQDEVTRTIVNALRVKLTAGEEERRESRGKVDPEAYDLLVRSRQAILQFNALS
SMEARRMLHRVLEIDPGMAAAHASLSIIALTDFINQWNGATPDNLTQALGLAQEAIDTDG
SEPQGHYTLALALSWMRRLDEAEHAAERAIELDPNSANAYTALGTIRDFQGRHEEALALY
TRAHRLDPQFDLSLHFQGRALLNLGRFDEAEVAFKRRLLLAPRSDMTRFYLACLYGRTGR
HEEARGYWREVLGVNPSFSVDHLRRSLPYQDPHLMDRLVEGLREAGVSI