Protein Info for SMa1548 in Sinorhizobium meliloti 1021

Annotation: Diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1071 TIGR00229: PAS domain S-box protein" amino acids 13 to 138 (126 residues), 47 bits, see alignment E=2.8e-16 amino acids 139 to 262 (124 residues), 60.3 bits, see alignment E=2.1e-20 amino acids 265 to 387 (123 residues), 93.9 bits, see alignment E=8.2e-31 amino acids 392 to 515 (124 residues), 52.7 bits, see alignment E=4.7e-18 amino acids 528 to 638 (111 residues), 34.7 bits, see alignment E=1.8e-12 PF13188: PAS_8" amino acids 16 to 70 (55 residues), 30 bits, see alignment 1.8e-10 amino acids 269 to 332 (64 residues), 22.3 bits, see alignment 4.7e-08 amino acids 396 to 453 (58 residues), 22.2 bits, see alignment 5.3e-08 amino acids 529 to 580 (52 residues), 18.1 bits, see alignment 1e-06 PF00989: PAS" amino acids 17 to 104 (88 residues), 32.9 bits, see alignment E=3e-11 amino acids 143 to 254 (112 residues), 35.1 bits, see alignment E=6.1e-12 amino acids 270 to 380 (111 residues), 50.4 bits, see alignment E=1.1e-16 amino acids 396 to 506 (111 residues), 28.7 bits, see alignment E=5.9e-10 PF08448: PAS_4" amino acids 23 to 133 (111 residues), 56.2 bits, see alignment E=1.9e-18 amino acids 159 to 259 (101 residues), 42 bits, see alignment E=4.8e-14 amino acids 276 to 385 (110 residues), 37.1 bits, see alignment E=1.7e-12 amino acids 401 to 511 (111 residues), 55.6 bits, see alignment E=3e-18 amino acids 534 to 634 (101 residues), 54.1 bits, see alignment E=8.7e-18 PF13426: PAS_9" amino acids 26 to 129 (104 residues), 38.2 bits, see alignment E=7.7e-13 amino acids 160 to 255 (96 residues), 25.2 bits, see alignment E=8.5e-09 amino acids 279 to 382 (104 residues), 29.4 bits, see alignment E=4e-10 amino acids 405 to 508 (104 residues), 36.9 bits, see alignment E=1.9e-12 amino acids 539 to 631 (93 residues), 22.5 bits, see alignment E=5.6e-08 PF08447: PAS_3" amino acids 167 to 250 (84 residues), 75.6 bits, see alignment 1.6e-24 amino acids 293 to 376 (84 residues), 64.9 bits, see alignment 3.3e-21 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 647 to 808 (162 residues), 135.5 bits, see alignment E=1.5e-43 PF00990: GGDEF" amino acids 650 to 804 (155 residues), 154.2 bits, see alignment E=1.3e-48 PF00563: EAL" amino acids 827 to 1056 (230 residues), 236.4 bits, see alignment E=1.5e-73

Best Hits

KEGG orthology group: None (inferred from 100% identity to sme:SMa1548)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92YN0 at UniProt or InterPro

Protein Sequence (1071 amino acids)

>SMa1548 Diguanylate cyclase/phosphodiesterase (Sinorhizobium meliloti 1021)
MNAPTPEPPSHRSHDLFQRLMDGIAEPVIIKDDASRFVFINDAACDLLGKDRNQLIGRTD
HDILPGEQADRIVSLDRIVLSTSEGHELEEQITTPDGTQRTLLTKKRCVSIPAGSTQEKF
VVVTIVDITNLRRTEETLRASEEHYRSLVDLHPQVPWTADTAGEVLEVGPRWSELTGLGE
KETLGSGWAKAVHPQDAGALQEEWRRSLASGAPFDCEYRLLTKTGDYRWFRARAAAKRDA
DGKIVRWYGVLEDIDERRRAADDLRESEARFRAIADDAPVMIWVADPTGDTSFFNRLWLE
TTGQTEAEALGFGWVDVIHPDDRQAVQETFFRATAGKEPVRSEYRLRRADGSWAWVIDVG
QPRFSADGTFLGYVGSVLDITERRAAEIAQQEAQAFIRSIFDSSPDCVRVLDMEGRPLLM
NEAGRRIFGLNEGAPVTGQTWDSIGRASDADKVEAAWESVRRGKTARFEISVRDAGGEER
CMDVISAPITDHHGKPFRILSIWRDITDAKRASDEISRAQRHAEAAADQLSSVLESTMDS
VMLLDADWRVRYLNENARKLLQVGDEALGRVFWKLFPEEEEGSFAKHCREVMDRRVRSFF
EDHLSSLGRWVEANASPTRDGISIFCRDITERRRAEEDTLLAQKQMAHMARHDMLTGLAN
RMFFRECFEEALNESNHARMAVLCLDLDGFKAVNDTLGHPAGDALLRQVSTRLIQAVRTT
ETVARLGGDEFAIIQPLTESRDEAFRLAQRLIDTLSEPFSIEGAAANVGTSIGIAFAPED
GTSADELIKAADIALYSAKSSGRGTYKLFDVAMHAQLQAHQQMKITMRDALAKGEFELHY
QPLVSLESRCVSCCEALLRWRHPERGMIAPSEFIPIAEETGLIVPIGEWILGEACRQAAR
WPERVSVAVNLSPVQFKHRNLVRAVAKALSATRLDPARLQLEITETVLLDESEHNLELLQ
DLRRLGVKIAMDDFGTGYSSLGYLRSFPFDKIKVDQAFVRDLPHGKESLAIVKAVAGLGQ
SLGMTTSVEGVETEDQLAVVDSEGFNEVQGYLFSRPLPAAEISKLIAAGSL