Protein Info for SMa1013 in Sinorhizobium meliloti 1021

Annotation: ATPase P

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 826 transmembrane" amino acids 172 to 190 (19 residues), see Phobius details amino acids 210 to 228 (19 residues), see Phobius details amino acids 246 to 268 (23 residues), see Phobius details amino acids 275 to 293 (19 residues), see Phobius details amino acids 427 to 449 (23 residues), see Phobius details amino acids 455 to 477 (23 residues), see Phobius details amino acids 771 to 790 (20 residues), see Phobius details amino acids 796 to 818 (23 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 18 to 78 (61 residues), 38.8 bits, see alignment 2.2e-13 amino acids 84 to 146 (63 residues), 47.9 bits, see alignment 3.1e-16 PF00403: HMA" amino acids 20 to 78 (59 residues), 57.4 bits, see alignment 3.9e-19 amino acids 86 to 145 (60 residues), 58.2 bits, see alignment 2.2e-19 TIGR01511: copper-translocating P-type ATPase" amino acids 225 to 821 (597 residues), 596.9 bits, see alignment E=1.1e-182 TIGR01525: heavy metal translocating P-type ATPase" amino acids 243 to 820 (578 residues), 638.8 bits, see alignment E=2.5e-195 TIGR01494: HAD ATPase, P-type, family IC" amino acids 282 to 789 (508 residues), 311.3 bits, see alignment E=2.4e-96 PF00122: E1-E2_ATPase" amino acids 311 to 491 (181 residues), 199.4 bits, see alignment E=9.2e-63 PF00702: Hydrolase" amino acids 509 to 725 (217 residues), 130.5 bits, see alignment E=2.6e-41 PF08282: Hydrolase_3" amino acids 699 to 757 (59 residues), 25.8 bits, see alignment 2.1e-09

Best Hits

Swiss-Prot: 100% identical to ATCU1_RHIME: Copper-transporting ATPase 1 (actP1) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to sme:SMa1013)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P58341 at UniProt or InterPro

Protein Sequence (826 amino acids)

>SMa1013 ATPase P (Sinorhizobium meliloti 1021)
MTAFTQIEKSAAVPAPTDFGIEGMTCASCVRRVEKAISAVPGVASATVNLATERASVQFT
GAPDTGGVLLAIEKAGYEPKVIIQEFGIEGMTCASCVSRVEKALRTVPGVADASVNLATE
KGTVRFVSGVDVAAIEAAVRDAGYDVRKAKASGATAEPEDRRELETRTLKRLVILSAVLT
LPLFLVEMGSHFMPGVHEWIMENIGMRHNLYIQFALATAVLFGPGLRFFRKGVPNLLRWT
PDMNSLVVLGTTAAWGYSVVATFASGLLPSGTANVYYEAAAVIVTLILLGRYLEARAKGR
TSQAIKRLLGLQPKTAFVAHGDEFVEIQISDVVVGDVIRIRPGEKIPVDGTVLDGNSYVD
ESMITGEPVPVQKAAGAEVVGGTINKNGSFTFRATKVGGDTLLAQIIKMVETAQGSKLPI
QALVDKVTAWFVPAVILVAVLTFAAWYVFGPSPALTFALVNAVAVLIIACPCAMGLATPT
SIMVGTGRAAELGILFRKGEALQSLREADVIALDKTGTLTKGRPELTDIVPADGFEADEV
LSFVASLEALSEHPIAEAIVSAAKSRGIALVPATDFEATPGFGVRGAVSGLPVQVGADRA
FSGVGIDVSPFVVEAERLGNSGKSPLYAAIDGRLAAIIAVSDPIKDTTPQAIKALHDLGL
KVAMITGDNRRTADAIARQLGIDEVVAEVLPDGKVDAVKRLREGGRKVAFIGDGINDAPA
LTEADVGIAVGTGTDIAIESADVVLMSGDLIGVPKAIALSKATIRNIKQNLFWAFAYNVS
LVPVAAGVLYPLNGTLLSPILAAAAMAMSSVFVLGNALRLRSVNPA