Protein Info for SMa0929 in Sinorhizobium meliloti 1021

Annotation: conjugal transfer protein TraG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 639 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 46 to 70 (25 residues), see Phobius details amino acids 77 to 95 (19 residues), see Phobius details amino acids 115 to 140 (26 residues), see Phobius details TIGR02767: Ti-type conjugative transfer system protein TraG" amino acids 10 to 634 (625 residues), 1091.9 bits, see alignment E=0 PF02534: T4SS-DNA_transf" amino acids 168 to 635 (468 residues), 336 bits, see alignment E=6e-104 PF10412: TrwB_AAD_bind" amino acids 436 to 596 (161 residues), 59.3 bits, see alignment E=4.9e-20 PF12696: TraG-D_C" amino acids 474 to 595 (122 residues), 88.7 bits, see alignment E=4.7e-29

Best Hits

Swiss-Prot: 56% identical to VIRD4_BARHE: Type IV secretion system-coupling protein VirD4 (virD4) from Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)

KEGG orthology group: K03205, type IV secretion system protein VirD4 (inferred from 100% identity to sme:SMa0929)

Predicted SEED Role

"Type IV secretion system protein VirD4" in subsystem Type 4 secretion and conjugative transfer or pVir Plasmid of Campylobacter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92ZI3 at UniProt or InterPro

Protein Sequence (639 amino acids)

>SMa0929 conjugal transfer protein TraG (Sinorhizobium meliloti 1021)
MALKAKPHPSLLVILFPVAVTAAAVYVVGWRWPGLAAGMSGKTAYWFLRAAPVPALLFGP
LAGLLAVWALPLHRRRPVAMASLACFLTVAGFYALREFGRLSPSVESGALSWDRALSYLD
MVAVVGAVVGFMAVAVSARISTVVPEPVKRAKRGTFGDADWLPMAAAGKLFPPDGEIVIG
ERYRVDKDIVHELPFEPNDPATWGQGGKAPLLTYRQDFDSTHMLFFAGSGGYKTTSNVVP
TALRYTGPLICLDPSTEVAPMVVEHRTRVLGREVMVLDPTNPIMGFNVLDGIEHSRQKEE
DIVGIAHMLLSESVRFESSTGSYFQNQAHNLLTGLLAHVMLSPEYAGRRTLRSLRQIVSE
PEPSVLAMLRDIQERSASTFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSSDIVSGKKDVFLNIPASILRSYPGIGRVIIGSLINAMIQADGSFKRRALFMLDE
VDLLGYMRLLEEARDRGRKYGISMMLLYQSLGQLERHFGRDGAVSWIDGCAFASYAAVKA
LDTARNISAQCGEMTVEVKGSSRNIGWDTKNSASRKSENVNYQRRPLIMPHEITQSMRKD
EQIIIVQGHSPIRCGRAIYFRRKDMNEAAKANRFVKAIP