Protein Info for SMa0878 in Sinorhizobium meliloti 1021

Annotation: glucosamine--fructose-6-phosphate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 608 (607 residues), 779.6 bits, E=1e-238
• PF13522: GATase_6
  • amino acids 65 to 180 (116 residues), 67.6 bits, E=1.8e-22
• PF13537: GATase_7
  • amino acids 82 to 196 (115 residues), 56.6 bits, E=4.1e-19
• PF01380: SIS
  • amino acids 292 to 414 (123 residues), 92.2 bits, E=3.8e-30
  • amino acids 459 to 589 (131 residues), 101.2 bits, E=6e-33

Best Hits

Swiss-Prot: 100% identical to NODM_RHIME: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (nodM) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 100% identity to smk:Sinme_6315)

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See Q92ZK3 at UniProt or InterPro

Protein Sequence (608 amino acids)

>SMa0878 glucosamine--fructose-6-phosphate aminotransferase (Sinorhizobium meliloti 1021)
MCGIVGIVGNQPVSERLVEALKRLEYRGYDSAGVATIDAGTLQRRRAEGKLVNLESRLRE
EPLAGTIGIAHTRWATHGAPTERNAHPHFTEGVAVVHNGIIENFAELKDELAAGGAEFQT
ETDTEVVAHLLTKYRRDGLGRREAMHAMLKRVKGAYALAVLFEDDPSTIMAARNGPPLAI
GHGSGEMFLGSDAIALAPFTNEITYLIDGDWAVIGKTGVHIFDFDGNVVERPRQISTAAA
FLVDKGNHRHFMEKEIYEQPEVIAHALGHYVNFIENRVVPISDAIDFGKVPSLAISACGT
AYLAGLIGKYWFERYARLPVEIDVASEFRYREIPLSPQSAALFISQSGETADTLASLRYC
KEHGLKIGAVVNARESTIARESDAVFPILAGPEIGVASTKAFTCQLAVLAALAVGAGKAR
GTISGEEEQALVKSLAEMPRIMGQVLNSIQPKIESLSRELSKCHDVLYLGRGTSFPLAME
GALKLKEISYIHAEGYAAGELKHGPIALIDENMPVIVIAPHDRFFDKTVSNMQEVAARGG
RIILITDEKGAAASKLDTMHTIVLPEVDEIIAPMIFSLPLQLLAYHTAVFMGTDVDQPRN
LAKSVTVE