Protein Info for SMa0873 in Sinorhizobium meliloti 1021

Annotation: nitrogenase molybdenum-cofactor biosynthesis protein NifN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 transmembrane" amino acids 21 to 39 (19 residues), see Phobius details amino acids 324 to 343 (20 residues), see Phobius details TIGR01285: nitrogenase molybdenum-iron cofactor biosynthesis protein NifN" amino acids 3 to 431 (429 residues), 625.8 bits, see alignment E=2.1e-192 PF00148: Oxidored_nitro" amino acids 19 to 429 (411 residues), 344 bits, see alignment E=5.4e-107

Best Hits

Swiss-Prot: 100% identical to NIFN_RHIME: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (nifN) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02592, nitrogenase molybdenum-iron protein NifN (inferred from 100% identity to smk:Sinme_6319)

MetaCyc: 40% identical to Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (Azotobacter vinelandii)
RXN-17026

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifN" in subsystem Nitrogen fixation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P12781 at UniProt or InterPro

Protein Sequence (441 amino acids)

>SMa0873 nitrogenase molybdenum-cofactor biosynthesis protein NifN (Sinorhizobium meliloti 1021)
MVRILSQTKWATINPLKSSQPLGGALAFLGVGGAIPLFHGSQGCTSFALVLLVRHFKEAI
PLQTTAMDDVAIVLGGAGHLEQAILNLKIRAKPKLIGICTTALVETRGEDLAGDLASIKL
ERAEELTGTDVVLANTPDFDGAMEEGWAKAVTAMIKAITRIGEQERQSRTIAILPGWNLT
IADIEQLRDIVESFGLKPIILPDLSGSLDGIVPDDRWVPTTYGGISVEEIRELGTAAQCI
AIGEHMRGPAEEMKTLTGVPYVLFQSLTGLNAVDRFVSLLSSISGRPAPAKVRRRRAQLQ
DALLDGHFHSAGKKIAIAAEPDQLYQLATFFICLGAEIVAAVTTKGASKILHKVPVEIIQ
VGDLGDLESLATHADLLVTHSHGQHASARLGTPLMRVGFPVFDQLGSQHKLTILYHGTRD
LIFEVSNIFQSHSLAPTHRGT