Protein Info for SMa0829 in Sinorhizobium meliloti 1021

Annotation: NifK nitrogenase molybdenum-iron protein beta chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 PF11844: DUF3364" amino acids 1 to 56 (56 residues), 78.5 bits, see alignment 3.5e-26 TIGR01286: nitrogenase molybdenum-iron protein beta chain" amino acids 3 to 513 (511 residues), 922.1 bits, see alignment E=3.9e-282 PF00148: Oxidored_nitro" amino acids 70 to 490 (421 residues), 406.7 bits, see alignment E=1e-125

Best Hits

Swiss-Prot: 87% identical to NIFK_SINFN: Nitrogenase molybdenum-iron protein beta chain (nifK1) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02591, nitrogenase molybdenum-iron protein beta chain [EC: 1.18.6.1] (inferred from 100% identity to smk:Sinme_6350)

MetaCyc: 57% identical to [FeMo]-nitrogenase complex dinitrogenase component beta subunit (Trichormus variabilis)
Nitrogenase. [EC: 1.18.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92ZL3 at UniProt or InterPro

Protein Sequence (513 amino acids)

>SMa0829 NifK nitrogenase molybdenum-iron protein beta chain (Sinorhizobium meliloti 1021)
MPQSAEKVLDHAPLFREPEYRKMLAEKKRNFERPYPDRTVTDQREFTKTWHYREINLARE
ALVVNPAKACQPLGAVFAAAGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTE
DAAVFGGLKNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLHGFIENAKDEGAVPHDFD
VPFAHTPAFVGSHVDGYDSMVKGVLENFWKGEQRTVNPGSINIIPGFDGFCVGNNRELKR
LLNLMGVSYTFIQDASDQFDTPSDGEFRMYDGGTKIEDVRAALNAEATVSLQQYNTRRTL
EYCKAAGQSTVSFHYPLGVKATDEFLVKVSEISGREIPEAIRLERGRLVDAMADSQSWLH
GKKYAIYGDPDFVYAVARFIMETGGEPTHCLATNGTPAWEAEMKMLLASSPLGNDAQVWA
SKDLWAMRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
LRVLTTILDKIFDRLDRETMQVGVTDYSYDLTR