Protein Info for SMa0819 in Sinorhizobium meliloti 1021

Annotation: FixB electron transfer flavoprotein alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF01012: ETF" amino acids 15 to 188 (174 residues), 145.5 bits, see alignment E=1.6e-46 PF00766: ETF_alpha" amino acids 220 to 300 (81 residues), 111.6 bits, see alignment E=1.4e-36

Best Hits

Swiss-Prot: 100% identical to FIXB_RHIME: Protein FixB (fixB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 100% identity to sme:SMa0819)

MetaCyc: 60% identical to quinone reductase (NADH,flavodoxin) complex electron transfer flavoprotein component beta subunit (Azotobacter vinelandii)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P09819 at UniProt or InterPro

Protein Sequence (353 amino acids)

>SMa0819 FixB electron transfer flavoprotein alpha chain (Sinorhizobium meliloti 1021)
MKKGLPKQFQDYRNVWVFIELEHGQVHPVSIELLGEGRKLADKLGVHLAGVVIGPPGGQG
TANAIADAFAYGADLSYLVESPLLAHYRNEPFTKALTDLVLANKPEILLLGATTLGRDLA
GSVATTLKTGLTADCTELNVDSDGSLAATRPTFGGSLLCTIYTLKCRPQMATVRPSVMAT
PQRVNRPTGSIIRHDLKMLEEEIATKVLAFFSDCDSTIANLAYADVVVAGGLGLGAVQNL
QLLKDLARTLGGDFGCSRPLVQKGWMPFDRQIGQTGNTIRPKLYIAAGISGAVQHRVGVE
GSDLIVAINTDPNAPIFDFAHLGVVADAISFLPALTEVFTKRLEPRNLEKFVQ