Protein Info for SMa0751 in Sinorhizobium meliloti 1021

Annotation: Ring-hydroxylating dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 43 to 126 (84 residues), 61.5 bits, E=5.8e-21
• PF00848: Ring_hydroxyl_A
  • amino acids 179 to 400 (222 residues), 143.4 bits, E=9.7e-46

Best Hits

KEGG orthology group: None (inferred from 100% identity to sme:SMa0751)

MetaCyc: 100% identical to stachydrine N-demethylase oxygenase subunit (Sinorhizobium meliloti 1021)
R501-RXN [EC: 1.14.13.247]

Predicted SEED Role

"Benzoate 1,2-dioxygenase (EC 1.14.12.10)" (EC 1.14.12.10)

MetaCyc Pathways

• proline betaine degradation I (2/3 steps found)
• benzoate degradation I (aerobic) (1/2 steps found)
• meta cleavage pathway of aromatic compounds (2/10 steps found)
• toluene degradation IV (aerobic) (via catechol) (2/13 steps found)
• mandelate degradation to acetyl-CoA (5/18 steps found)
• superpathway of aerobic toluene degradation (9/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (10/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (7/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10 or 1.14.13.247

Sequence Analysis Tools

See Q92ZP9 at UniProt or InterPro

Protein Sequence (412 amino acids)

>SMa0751 Ring-hydroxylating dioxygenase (Sinorhizobium meliloti 1021)
MTANPTSIHQRLDRRLSGFSLEQPFYTSPEVYALDLQHIFYKQWLYAVPVCQLAKAGSYT
TLRVGAYEVVIVRSRDGEVRAFHNSCRHRGSLICKARQGQVAKLVCPYHQWTYELDGKLI
WANDMGPDFDASKYGLKPVNLRNLDGLIYICLSDTPPDFQTFAQLARPYLEVHDLKDAKV
AFTSTIIEKGNWKLVWENNRECYHCSSNHPALCRSFPLDPEVAGVQADGGVSKKLQAHFD
RCEAAGTPAQFVLAGDGQYRLARMPLQEKALSYTMDGKAAVSRHLGRVAPPDAGTLLMFH
YPSTWNHFLPDHSLTFRVMPISPTETEVTTTWLVHKDAVEGVDYDLKRLTEVWIATNDED
REIVETNQQGILSPAYVPGPYSPGQESGVMQFVDWYAASLERALAPRQVAAE