Protein Info for SMa0621 in Sinorhizobium meliloti 1021

Annotation: FixI2 E1-E2 type cation ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 755 transmembrane" amino acids 121 to 142 (22 residues), see Phobius details amino acids 150 to 172 (23 residues), see Phobius details amino acids 184 to 203 (20 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 366 to 386 (21 residues), see Phobius details amino acids 397 to 421 (25 residues), see Phobius details amino acids 691 to 708 (18 residues), see Phobius details amino acids 714 to 732 (19 residues), see Phobius details PF00403: HMA" amino acids 43 to 100 (58 residues), 35.3 bits, see alignment 1.9e-12 TIGR01511: copper-translocating P-type ATPase" amino acids 168 to 733 (566 residues), 503.5 bits, see alignment E=1.7e-154 TIGR01525: heavy metal translocating P-type ATPase" amino acids 186 to 733 (548 residues), 482.5 bits, see alignment E=4.3e-148 TIGR01512: cadmium-translocating P-type ATPase" amino acids 209 to 733 (525 residues), 327.6 bits, see alignment E=2.6e-101 TIGR01494: HAD ATPase, P-type, family IC" amino acids 220 to 714 (495 residues), 228.8 bits, see alignment E=2e-71 PF00122: E1-E2_ATPase" amino acids 248 to 427 (180 residues), 138.8 bits, see alignment E=2.2e-44 PF00702: Hydrolase" amino acids 444 to 646 (203 residues), 103.8 bits, see alignment E=2.5e-33

Best Hits

KEGG orthology group: K01529, [EC: 3.6.1.-] (inferred from 100% identity to sme:SMa0621)

Predicted SEED Role

"Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.3.4

Use Curated BLAST to search for 3.6.1.- or 3.6.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92ZX3 at UniProt or InterPro

Protein Sequence (755 amino acids)

>SMa0621 FixI2 E1-E2 type cation ATPase (Sinorhizobium meliloti 1021)
MSCCSGIAVPSAGAASRTSISPEEIRLASRDLGDGLRQTAMTLPDAHCAACIAAVEGALR
KISGVELARVNLSARRVTINWRGNDDESPDFAAALAKIGYASHLASIEEETQDPVLASLL
KALAVAGFSAMNIMILSVSVWSGADPATRHAFHLVSAALALPAIVYSGRFFYRSAWAALR
HGRTNMDVPISVGVLLAFALSVYDTLHNAAFAYFDASTSLLFVLLAGRTLDHLMRGRARS
AVGALARLSPRGASVVQADEAIDYVPLSEIQPGMRLLVAAGERVPVDGVVVKGASELDAS
IVSGESEWRRAAPGSALQAGVMNLANPLTLLATASVDGSFLAEMTRMMEAAESGRSTYRR
IADRAASLYAPVVHGVALLSMVAWLFGTGDLHKSVTIAIAVLVITCPCALGLAVPMVQVV
AVRRLFERGIMARDGSAFERLNEIDTVLFDKTGTLTLGEMRLVNAGDIQPRLLSLAAAMA
RVSRHPASVAIALADPRRPVAPVEFDSLEEVHGCGIEGRAGDAVYRLGRPSWASTAKQVD
LGTSSTTVLSKDAETIAVFAFEETVRPGARELVQTLRSAGLSVRILSGDRSAAVSSIARQ
LDIEAFSAELLPGEKVEAIRALAATGRKVLMIGDGLNDAPALAAAHVSIAPSSATDVGRS
ASDFVFLGQSLLAVRDIIQTAARADVLIRQNFAMAIAYNVVSVPFAIGGVVTPLAAALAM
SLSSIVVVGNALRQGAKVKKGPRTFGATKPATAKI