Protein Info for SMa0398 in Sinorhizobium meliloti 1021

Annotation: histidinol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 PF00815: Histidinol_dh" amino acids 36 to 445 (410 residues), 443.1 bits, see alignment E=5.6e-137 TIGR00069: histidinol dehydrogenase" amino acids 49 to 445 (397 residues), 414.5 bits, see alignment E=2.6e-128

Best Hits

Swiss-Prot: 100% identical to HISX2_RHIME: Histidinol dehydrogenase 2 (hisD2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 100% identity to sme:SMa0398)

Predicted SEED Role

"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.23

Use Curated BLAST to search for 1.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q930I4 at UniProt or InterPro

Protein Sequence (456 amino acids)

>SMa0398 histidinol dehydrogenase (Sinorhizobium meliloti 1021)
MTLRPQLARYKDKTMTSVSFYEYSKLNAEEKAALLRRSETDISGFIEKVAPILEAVRTEG
DKALARFGRELDKADVTEANLKVTAAEFDAAFKLVDASVLESVQFGIDNIRKFHEEQKPE
AMWLKEIRPGAFAGDRFTPIQSVALYVPRGKGSFPSVTMMTSVPAVVAGVPNLAIVTPPA
PDGSVDAATLVAARLAGVETVYKAGGAQAVAAVAYGTETVKPALKIVGPGSPWVVAAKRS
LSGVIDTGLPAGPSEVMILADDTVHGGLAALDLLIEAEHGPDSSAYLVTHSGRVAEEALA
ALPEHWARMTEQRTAFSKTVLSGKTGGIVLTSSIEESYEFVNAYAPEHLELLSEQPFIHL
GHITEASEILMGTHTPVSIANFSLGPNAVLPTSRWARTFGPLSVTDFVKRSSIGYVTAPA
YPEFARHSHNLAIYEGFSSHALAVSPVRDAYLKKGA