Protein Info for SMa0228 in Sinorhizobium meliloti 1021

Annotation: glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 57 to 184 (128 residues), 173.1 bits, E=2.1e-55
• PF00208: ELFV_dehydrog
  • amino acids 203 to 446 (244 residues), 306.8 bits, E=1.2e-95

Best Hits

Swiss-Prot: 66% identical to DHE4_CORGL: NADP-specific glutamate dehydrogenase (gdh) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 100% identity to smk:Sinme_5372)

MetaCyc: 59% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.4

Sequence Analysis Tools

See Q930S3 at UniProt or InterPro

Protein Sequence (448 amino acids)

>SMa0228 glutamate dehydrogenase (Sinorhizobium meliloti 1021)
MNVDEKLEPILAEVLRRNGGEHEFHQAVREVLESLGRVIAKHPRYAENALIERICEPERQ
IIFRVPWVDDKGQVQINRGFRVQFNSALGPYKGGIRFHPSVNIGIIKFLGFEQTFKNALT
GMPIGGGKGGSDFNPRGRSDGEIMRFCQSLMTELHRHLGEYTDVPAGDIGVGGREIGYMF
GQYKRLTNRYEAGVLTGKALFYGGSRARKEATGYGATYFVQRMIATKGLDFEGKRVTVSG
SGNVAIYTMEKVIEFGGKIVACSDSNGYVVDEDGIDLELVKEIKEVRRERISEYARLKGA
GTHYIEAGSVWDVPCDVAMPSATQNELTGKDARTLVKNGVLAVGEGANMPCTPEAVRIFQ
EAGVLFAPGKAANAGGVATSALEMQQNASRDSWTFEQTEARLATIMQAIHDRCAETAEEY
GTPGDYVLGANIAGFVRVAEAMDALGVI