Protein Info for SM_b21488 in Sinorhizobium meliloti 1021
Annotation: cytochrome o ubiquinol oxidase subunit I
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 67% identical to CYOB_ECOL6: Cytochrome bo(3) ubiquinol oxidase subunit 1 (cyoB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K02298, cytochrome o ubiquinol oxidase subunit I [EC: 1.10.3.-] (inferred from 84% identity to avi:Avi_6215)MetaCyc: 67% identical to cytochrome bo3 subunit 1 (Escherichia coli K-12 substr. MG1655)
RXN-21817 [EC: 7.1.1.3]
Predicted SEED Role
"Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)
MetaCyc Pathways
- NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (2/2 steps found)
- pyruvate to cytochrome bo oxidase electron transfer (2/2 steps found)
- succinate to cytochrome bo oxidase electron transfer (2/2 steps found)
- D-lactate to cytochrome bo oxidase electron transfer (1/2 steps found)
- NADH to cytochrome bo oxidase electron transfer II (1/2 steps found)
- proline to cytochrome bo oxidase electron transfer (1/2 steps found)
Isozymes
Compare fitness of predicted isozymes for: 1.10.3.-
Use Curated BLAST to search for 1.10.3.- or 7.1.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q92U26 at UniProt or InterPro
Protein Sequence (669 amino acids)
>SM_b21488 cytochrome o ubiquinol oxidase subunit I (Sinorhizobium meliloti 1021) MFSDPDFLKLIFGRLTLDAIPYHEPILVVTFLAVALGGIALIGLITYYRFWGTLWRDWLT SVDHKKIGIMYIILALVMLLRGFSDAIMMRLQQAMAFGGSEGYLPPHHYDQVFTAHGVIM IFFMAMPFVTGLMNFVVPLQIGARDVSFPFLNNFSFWMTTAGAIIIMLSLFVGEFARTGW LAYPPLSGADYSPGVGVDYYVWGLQIAGIGTTLSGINLIATIVKMRAPGMTMMKMPVFTW TSLCTNVLIVATFPVLTATLALLTLDRYAGTNFFTNDLGGNPMMYVNLIWIWGHPEVYIL ILPAFGIFSEVVATFCGKRLFGYASMVYATVVITILSYIVWLHHFFTMGSGASVNAFFGI TTMIISIPTGAKMFNWLFTMYRGRIRYEVPMLWTIGFMVTFVLGGMTGVLLAVPPADFVL HNSLFLIAHFHNVIIGGVVFGLMAGVVYWFPKAFGFRLDPFWGKMSFWFWLTGFYFAFMP LYVLGLMGVTRRVSQFEDPSLQIWFVIAAFGAFLIALGILSMLIQLVVSFVRREELRDTT GDPWDGRTLEWSTASPPPDYNFAFTPLVHDQDSWWDMKKRGHSRPLVGFKPIHMPKNTGT GVILGAISVVFAFAMVWYMWWLVILSFVAMAAIAIGHTFNYDRDFHIPANVVADTEGKRT EALAPEVQA