Protein Info for SM_b21438 in Sinorhizobium meliloti 1021

Annotation: C4-dicarboxylate transport system, C4-dicarboxylate-binding protein precursor signal peptide

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF03480: DctP" amino acids 37 to 314 (278 residues), 301.9 bits, see alignment E=2.3e-94 TIGR00787: TRAP transporter solute receptor, DctP family" amino acids 37 to 292 (256 residues), 271.5 bits, see alignment E=3.5e-85

Best Hits

Swiss-Prot: 35% identical to YIAO_ECOLI: 2,3-diketo-L-gulonate-binding periplasmic protein YiaO (yiaO) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to sme:SM_b21438)

MetaCyc: 35% identical to 2,3-diketo-L-gulonate:Na+ symporter - periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-235

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, periplasmic component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q926B4 at UniProt or InterPro

Protein Sequence (332 amino acids)

>SM_b21438 C4-dicarboxylate transport system, C4-dicarboxylate-binding protein precursor signal peptide (Sinorhizobium meliloti 1021)
MKLGISKISRGLAATVIAGIVGLSAHSAAMAETTIRLGHVLADTHSWHKASTDFAKEVSE
KTEGRVKIEIFPSGQLGTEKEVIEGMQIGTIQGGLIGSGSFQFVEPKFGIIEMPYAWTSR
EQAFAALDGKLGTALADLLRPKGIEVLAWWENGFRNITNNKHPIVKPADLAGIKIRVTPD
KVRLATFERLGAQPAPLAFGELYSALQQGVFDAQENPLSIIDASSFYEVQKYVSMTGHIW
GAACLTVSSMTWAQVSPEDQAIVKEAAVKWGNAQRQMVADNEVALIEKLKSKGMQFNEVD
KAAFVDALKPIWESEKDVFGPELLEVMDSYRK