Protein Info for SM_b21428 in Sinorhizobium meliloti 1021

Annotation: dTDP-4-dehydrorhamnose 3,5-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01221: dTDP-4-dehydrorhamnose 3,5-epimerase
  • amino acids 17 to 184 (168 residues), 159.5 bits, E=3.6e-51
• PF00908: dTDP_sugar_isom
  • amino acids 17 to 183 (167 residues), 199.4 bits, E=1.8e-63

Best Hits

Swiss-Prot: 39% identical to RMLC_METTH: dTDP-4-dehydrorhamnose 3,5-epimerase (rmlC) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

KEGG orthology group: K01790, dTDP-4-dehydrorhamnose 3,5-epimerase [EC: 5.1.3.13] (inferred from 100% identity to sme:SM_b21428)

Predicted SEED Role

"dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13)" in subsystem Capsular heptose biosynthesis or Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 5.1.3.13)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.13

Use Curated BLAST to search for 5.1.3.13

Sequence Analysis Tools

See Q92U81 at UniProt or InterPro

Protein Sequence (187 amino acids)

>SM_b21428 dTDP-4-dehydrorhamnose 3,5-epimerase (Sinorhizobium meliloti 1021)
MKCSLGGLMSRFSRLSTPLAGLTVIERKQMGDERGFFSRFFCRDELRDFGADGTISQINH
TLTRAKGTIRGMHFQRPPHDEAKFVSCLAGAVFDVAVDIRPDSPTYLQWHGEILSGENAR
SMMIPGGFAHGFQTLSENCELVYLHDKPYAPDAEGGLNPLDPRLAISWPLEVAQMSERDR
AFAFISA