Protein Info for SM_b21416 in Sinorhizobium meliloti 1021

Annotation: glucose-1-phosphate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 255 TIGR02623: glucose-1-phosphate cytidylyltransferase" amino acids 1 to 254 (254 residues), 431.1 bits, see alignment E=6.8e-134 PF00483: NTP_transferase" amino acids 2 to 203 (202 residues), 58.4 bits, see alignment E=8.3e-20 PF12804: NTP_transf_3" amino acids 3 to 57 (55 residues), 32.3 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 63% identical to RFBF_SALTI: Glucose-1-phosphate cytidylyltransferase (rfbF) from Salmonella typhi

KEGG orthology group: K00978, glucose-1-phosphate cytidylyltransferase [EC: 2.7.7.33] (inferred from 100% identity to smk:Sinme_4481)

MetaCyc: 61% identical to alpha-D-glucose-1-phosphate cytidylyltransferase monomer (Yersinia pseudotuberculosis)
Glucose-1-phosphate cytidylyltransferase. [EC: 2.7.7.33]

Predicted SEED Role

"Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33)" in subsystem dTDP-rhamnose synthesis (EC 2.7.7.33)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.33

Use Curated BLAST to search for 2.7.7.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92U93 at UniProt or InterPro

Protein Sequence (255 amino acids)

>SM_b21416 glucose-1-phosphate cytidylyltransferase (Sinorhizobium meliloti 1021)
MKVVILAGGYGTRISEESHLRPKPMIEIGGRPILWHIMKIYSHYGFSDFVICLGYRGYMI
KEYFSNYVLHSSDVTFDMATGETAYHTKSAEPWRVTLVDTGPESMTGGRLKRVAGYLGDT
FCLTYGDGVADVDVRALTEFHLRHGREATVTSVVPPGRYGALATEAGQVMSFTEKPAGDN
GRINGGFFVLNRSVLDRIAGDDVAFESAPLEGLARDRQLMAFPHDGFWRPMDTLRDKTQL
EELWQQNRAPWKIWA