Protein Info for SM_b21374 in Sinorhizobium meliloti 1021

Updated annotation (from data): tagatose kinase (EC 2.7.1.101)
Rationale: Important on tagatose. This is the first step in tagatose catabolism.
Original annotation: sugar kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 52% identical to TAGK_AGRFC: Tagatose kinase (Atu3166) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4759)

MetaCyc: 52% identical to tagatose kinase (Agrobacterium fabrum C58)
Tagatose kinase. [EC: 2.7.1.101]

Predicted SEED Role

"Fructokinase (EC 2.7.1.4)" in subsystem Fructose utilization or Mannitol Utilization or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.7.1.4)

MetaCyc Pathways

D-sorbitol degradation I (3/3 steps found)
sucrose degradation II (sucrose synthase) (4/5 steps found)
superpathway of anaerobic sucrose degradation (14/19 steps found)
D-altritol and galactitol degradation (3/4 steps found)
sucrose degradation III (sucrose invertase) (3/4 steps found)
sucrose degradation IV (sucrose phosphorylase) (3/4 steps found)
heterolactic fermentation (13/18 steps found)
mannitol cycle (3/5 steps found)
sucrose degradation I (sucrose phosphotransferase) (1/3 steps found)
sucrose degradation VII (sucrose 3-dehydrogenase) (1/4 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.4

Use Curated BLAST to search for 2.7.1.101 or 2.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92UY2 at UniProt or InterPro

Protein Sequence (313 amino acids)

>SM_b21374 tagatose kinase (EC 2.7.1.101) (Sinorhizobium meliloti 1021)
MHKILTIGEILVEIIATEKGDGFRKATPLIGPFPSGAPAIFIDQAGKLGQPCAIISRVGG
DDFGTVNLERLKRDGVDISGIEVDPLATTGSAFVRYRPDGSRAFVFNIRDSACGKITLDE
RMTRLVGECSHVHVMGSSLYAPSVVESILAAIGIVKAGGGTVSFDPNLRPEILKSPGMRE
ALLTVLAETDLFLPSGDELFLFTEAKTESQAVAELLASGIKAVVVKRGAAGASYFDAGAA
LSLPGFPVEEIDPTGAGDCFGATFVSFWLNGASPREALEFAAASGARAVMHFGPMEGAST
RAELERFISEQQA