Protein Info for SM_b21349 in Sinorhizobium meliloti 1021

Annotation: 5-keto-4-deoxyuronate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to KDUI2_RHIME: 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase 2 (kduI2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01815, 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase [EC: 5.3.1.17] (inferred from 100% identity to smk:Sinme_4783)

MetaCyc: 47% identical to 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase subunit (Dickeya dadantii 3937)
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase. [EC: 5.3.1.17]

Predicted SEED Role

"4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 5.3.1.17)

MetaCyc Pathways

4-deoxy-L-threo-hex-4-enopyranuronate degradation (5/5 steps found)
superpathway of microbial D-galacturonate and D-glucuronate degradation (19/31 steps found)

KEGG Metabolic Maps

Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.17

Use Curated BLAST to search for 5.3.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92V09 at UniProt or InterPro

Protein Sequence (282 amino acids)

>SM_b21349 5-keto-4-deoxyuronate isomerase (Sinorhizobium meliloti 1021)
MTASHIDYTIRYAVDPAAAARMDTDALRANFHIGDLFRQGRISLTYSHYDRMIVGGAMPV
DKPLALETIRPTGTARFLERRELIAVNIGGPGRIEMNGESFRLEPRDMAYAGMGEDVTFS
SEDPAVPAKFYLLSAPAHQALPRRHIRIGDAKRLDLGSAATSNERSIFQFIHPEGVKTCQ
LVVGMTQLAPGSVWNTMPCHVHDRRMEAYLYFDLDDSARVLHLMGEPSETRHIVMAREEA
VLSPPWSIHSGCGTANYAFIWAMAGDNIDYTDVEMVPMETLR