Protein Info for SM_b21293 in Sinorhizobium meliloti 1021

Annotation: guanine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02967: guanine deaminase
  • amino acids 26 to 426 (401 residues), 599 bits, E=2.2e-184
• PF01979: Amidohydro_1
  • amino acids 67 to 428 (362 residues), 176.6 bits, E=8.5e-56
• PF07969: Amidohydro_3
  • amino acids 102 to 428 (327 residues), 44.1 bits, E=2.1e-15

Best Hits

Swiss-Prot: 37% identical to GUAD_DEIRA: Probable guanine deaminase (guaD) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4908)

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3)" in subsystem Purine Utilization or Purine conversions (EC 3.5.4.3)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3

Use Curated BLAST to search for 3.5.4.3

Sequence Analysis Tools

See Q92VB1 at UniProt or InterPro

Protein Sequence (447 amino acids)

>SM_b21293 guanine deaminase (Sinorhizobium meliloti 1021)
MSDQLIRGRVLTFVKEPAGVDDAGSYRFFEDGAVFVSKGKIAGVGPYGDVAKHVGPSVKI
ADHRPHLVMPGFIDTHLHFPQTQAIASYGAQLLEWLNTYIFVEEQKFKAPEHAAFIADRF
MDELLSNGTTTAAAYCSVHPESVDAFFTAAEERNMLMIGGKVMMDRNAPDALRDTPEKGY
DETKALIAKWHGRGRAHYAISPRFAITSTPEQMEVSRVLAAEHPDCYVQTHLSENRDEIA
FATSLYPEAKDYTDIYARYELLGRKTLLGHCIHLSDREISVLAKTGAVGVFCPTSNLFLG
SGLFDRDRFDRLGARHAVATDVGGGTSFSMLETMDEAYKVLHLQGQRLSPLNSFYMLTLG
NARALDLEHRIGSLHAGADADIVVLDSRAKPAMELRMQVASTLAEELFVLQTMGDDRSVA
EVYVAGKPMKSGRRETKAEAVRALAMA