Protein Info for SM_b21284 in Sinorhizobium meliloti 1021

Annotation: uricase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 TIGR03212: putative urate catabolism protein" amino acids 3 to 298 (296 residues), 531.8 bits, see alignment E=3.9e-164 PF01522: Polysacc_deac_1" amino acids 73 to 162 (90 residues), 40.7 bits, see alignment E=2.2e-14 PF09349: OHCU_decarbox" amino acids 308 to 466 (159 residues), 166.4 bits, see alignment E=7.5e-53 TIGR03164: OHCU decarboxylase" amino acids 310 to 467 (158 residues), 215.9 bits, see alignment E=3.2e-68

Best Hits

KEGG orthology group: K00365, urate oxidase [EC: 1.7.3.3] (inferred from 100% identity to sme:SM_b21284)

Predicted SEED Role

"Uricase (urate oxidase) (EC 1.7.3.3) / 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase" (EC 1.7.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.3.3

Use Curated BLAST to search for 1.7.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92VC0 at UniProt or InterPro

Protein Sequence (471 amino acids)

>SM_b21284 uricase (Sinorhizobium meliloti 1021)
MRYPRDLLGHGPNPRIAWPDGARIAVQFVINYEEGGENCVLHGDAASEAFLSEIVGAQAW
PAQRHWNMESIYEYGARAGFWRLHRLFTDRQIPATVYGVATALKRSPAQVAAMQDAGWEI
ASHGLKWIEHKDFDAERERAEIAEAIRLHTIVTGERPTGWYTGRCSVNTLDLVTEAGGFD
YVSDAYADDLPYWHEHAGRHQLVIPYTLDANDMRFATPQGFNSGDQFFSYLKDSFDVLYA
EGTAGAPKMMSIGLHCRLVGRPGRAAALARFLDYVQSHEKVWLARRIDIARYWAETYPFQ
PNEDRPSRLSKDAFIDRFGGVFEHSDWIARRAFAGELGPANDTATGLHAALCAVFREASE
EERLAVLTAHPDLAGKLAQAKRLTESSTSEQASAGLDALTDQERERFTTLNNAYVEKFGF
PFILAVKGRSKDEILAAFENRIGNDRETEFVTACRQVERIALLRLRDTLPD