Protein Info for SM_b21232 in Sinorhizobium meliloti 1021

Annotation: nucleotide sugar epimerase dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 transmembrane" amino acids 30 to 53 (24 residues), see Phobius details amino acids 65 to 87 (23 residues), see Phobius details amino acids 99 to 119 (21 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details PF13727: CoA_binding_3" amino acids 83 to 236 (154 residues), 27 bits, see alignment E=1.3e-09 PF04321: RmlD_sub_bind" amino acids 324 to 455 (132 residues), 47.7 bits, see alignment E=3.4e-16 PF01370: Epimerase" amino acids 325 to 540 (216 residues), 63.2 bits, see alignment E=7.4e-21 PF02719: Polysacc_synt_2" amino acids 325 to 612 (288 residues), 359.5 bits, see alignment E=3.8e-111 PF16363: GDP_Man_Dehyd" amino acids 326 to 451 (126 residues), 45.8 bits, see alignment E=1.8e-15 PF01073: 3Beta_HSD" amino acids 326 to 454 (129 residues), 25.7 bits, see alignment E=1.7e-09

Best Hits

KEGG orthology group: K13013, O-antigen biosynthesis protein WbqV (inferred from 100% identity to sme:SM_b21232)

Predicted SEED Role

"UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-)" in subsystem N-linked Glycosylation in Bacteria (EC 4.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-

Use Curated BLAST to search for 4.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92VG8 at UniProt or InterPro

Protein Sequence (700 amino acids)

>SM_b21232 nucleotide sugar epimerase dehydratase (Sinorhizobium meliloti 1021)
MSGLLGATPALDIGFRRIRGAVRSVQKYPALYLTDLASVGVALAMALLLRYGVEELSARP
ETASVLLWSGAQYLTICALVFPLSGLYSRNWKYGSVSDLFIILRAVLLTSLLLVSLLFFS
TRLTDIPRTVVPMQSLLLIAFLAAARLSFRAEELALRRPVFKSGRSRDAQDDNRIPLLLI
GASDAADLYLRALARDPNATYTPVACLDRSEDQTGMSLRGVPIAGRIQDFERVVAELQQQ
GKQPRHIVFTEAPAAFGEEASDGLLRSAERLGIAVSRLSQMTELKRAKGDNPYELRSIEL
TDLLERPQAALDREAIGRLVRGRRVLITGAGGSIGSELTAQVAACEPAEIVLIDNTEYNL
YAIDMTLTESFPELPRWSYLCSVRRSQRVEEIFERHRPELVFHAAALKHVPMVQMNPCEG
VLTNVVGTMNVANAAKKYGTLAMVQVSTDKVVNSTSVMGATKRLAELYCQALDLNGLETG
LGPRFMTVRFGNVLGSSGSLIPLFKRQLARGGPLTVTDANMTRFFMTIREAVELTLQASA
YGFEKQLGQGEIFVLDMGEPIKIIDIARRMIRLAGFTPDQEIEIKIIGCRPGEKLFEELF
DETDKRTSSPVPGVLGAVPEPIPLPTLRDAFARLQRHSERGNEAGVVAVMRELLPRYEHE
ADRKMAAASKRTPLRPKSRIKAKEAKSPRGYRAEFRLPGR