Protein Info for SM_b21228 in Sinorhizobium meliloti 1021

Annotation: nucleotide sugar epimerase dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 293 (293 residues), 66.6 bits, E=8.4e-22
• PF05368: NmrA
  • amino acids 4 to 48 (45 residues), 23.4 bits, 1.5e-08
• PF01370: Epimerase
  • amino acids 4 to 230 (227 residues), 159.8 bits, E=3.2e-50
• PF01073: 3Beta_HSD
  • amino acids 4 to 219 (216 residues), 52.3 bits, E=1.8e-17
• PF13460: NAD_binding_10
  • amino acids 7 to 168 (162 residues), 50.3 bits, E=1.1e-16
• PF07993: NAD_binding_4
  • amino acids 61 to 212 (152 residues), 29.1 bits, E=2.3e-10
• PF16363: GDP_Man_Dehyd
  • amino acids 61 to 251 (191 residues), 50.3 bits, E=1e-16
• PF02719: Polysacc_synt_2
  • amino acids 61 to 252 (192 residues), 21.7 bits, E=4.1e-08

Best Hits

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4968)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• stachyose degradation (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• colanic acid building blocks biosynthesis (6/11 steps found)
• UDP-sugars interconversion (4/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Sequence Analysis Tools

See Q92VH2 at UniProt or InterPro

Protein Sequence (311 amino acids)

>SM_b21228 nucleotide sugar epimerase dehydratase (Sinorhizobium meliloti 1021)
MRLVVTGATGFIGARLIDRALSRGYEVTALARDPERAAARIGARLGVEKWSIGDPLPPLA
QSDAVLHLAAYIPADLSDARQAARCFEINTNGAIQIAMEAASQGVKRFVLFGSAQVYAPG
TEPASEASPAYPVHRASYYLASKLAAEICVLAFGKSSAMPVTVLRLASVYGPGMHDTGMV
PTFVRTLGSGRSLVIQDGGRYSVDLVYVDDVVTLALQAVETAEGGVFNAGSGRACTSLEA
AQIVADAVGAERHLIAVEGDSTDAAPRGFRVLNVSKAANELSYAPLSFREGIEAWKSMTG
LTDFQDGSVNA