Protein Info for SM_b21224 in Sinorhizobium meliloti 1021

Annotation: bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 22 to 210 (189 residues), 142.3 bits, E=2.7e-45
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 25 to 430 (406 residues), 629.9 bits, E=3.1e-193
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 442 to 624 (183 residues), 249.2 bits, E=3.4e-78
• PF01583: APS_kinase
  • amino acids 460 to 611 (152 residues), 246.4 bits, E=2.5e-77
• PF13671: AAA_33
  • amino acids 462 to 574 (113 residues), 29.5 bits, E=1.7e-10

Best Hits

Swiss-Prot: 99% identical to NODQ_RHIME: Bifunctional enzyme NodQ (nodQ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 100% identity to sme:SM_b21224)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (3/12 steps found)
• superpathway of seleno-compound metabolism (7/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.25, 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See Q92VH6 at UniProt or InterPro

Protein Sequence (633 amino acids)

>SM_b21224 bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase (Sinorhizobium meliloti 1021)
MSYVQSIPPHDIEAHLAEHDNKSILRFITCGSVDDGKSTLIGRLLYDAKLVFEDQLANLG
RVGSPGAANGKEIDLALLLDGLEAEREQGITIDVAYRYFATSKRKFIVADTPGHEEYTRN
MVTGASTADLAIILIDSRQGILQQTRRHSYIASLLGIRHVVLAVNKIDLVDFQQQVYEEI
VADYMAFAKELGFASIRPIPISARDGDNVISASANTPWYRGAALLEYLETVELDPTDQAK
PFRFPVQMVMRPNADFRGYAGQISCGRISVGDPVVVAKTGQRTSVKAIVTYDGELATAGE
GEAVTLVLSDEVDASRGNMLVAPGARPFVADQFQAHVIWFDANPMMPGRSYILRTETDSV
SATVTTLKHQVNINSFIREAAKSLQMNEVGVCNISTQAPIAFDAYKDNRATGNFIIVDRV
TNATVGAGLIDFPLRRADNVHWHALEVNKSARSAMKNQLPAVLWFTGLSGSGKSTIANEL
DRILHAQGKHTYLLDGDNVRHGLNRDLGFTEEDRVENIRRVAEVAKLMADAGLIVLVSFI
SPFRDERRMARELMEEGEFIEIFVDTPLDECARRDPKGLYEKALAGKIANFTGVSSPYEP
PENPELHIRTVGHEPTDLALAIEEFLDRRMEDK