Protein Info for SM_b21201 in Sinorhizobium meliloti 1021

Annotation: two-component sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 606 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 290 to 312 (23 residues), see Phobius details PF02743: dCache_1" amino acids 31 to 277 (247 residues), 60.6 bits, see alignment E=2.4e-20 PF00512: HisKA" amino acids 381 to 445 (65 residues), 28.8 bits, see alignment E=1.5e-10 PF02518: HATPase_c" amino acids 491 to 596 (106 residues), 75.7 bits, see alignment E=6.1e-25

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to sme:SM_b21201)

Predicted SEED Role

"MISCELLANEOUS; Not classified regulator"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92V51 at UniProt or InterPro

Protein Sequence (606 amino acids)

>SM_b21201 two-component sensor histidine kinase (Sinorhizobium meliloti 1021)
MKYRFVLALLLLPAAAGLIAVQGTAVATRSYMQEASSQANTALRLAVAALSGHLNRYQAL
PALIADHDDVKELVTRPRDRRLRGAVNSYLKEINELLKSSDIYVITPDGNTIAASNYDGP
TSFVGENFSYRPYFQDALKGDQSRFYALGTTSLKRGYYFGAPVRVGGEIRGVIVFKVDIE
TIEASWQGGEYRIFVSDPEGIIFMSGNPAWLYAGILPLTPDRLARTEGSRRYADARLRPL
PAVQSELSGHRLLRVSEGSEREYLALSHYMPEADWTVNVLTDTASVRTQALTTVAAVMLL
LCLAALAIAIIIQRRTRLRERILMQEQAQEELERRVEERTADLARVNSQIEAEIAERRLT
EQQLRQTQADLIQAGKLAGLGQMSAALSHEFNQPLAAAKTYADSAALLIERGRTAEASDN
IRRISGLIDRMASISRHLRNFARKPNEKLGPVRLDEVVRDTLEIVETRLKAAGAEMDIDI
GDELAVLAGSVRLQQVLVNVISNAADAVEGLDDRKLSVRAWREGGRAVLTVRDRGAGVAP
AVAQRIFDPFYTTKGVGRGLGLGLSISYNIIKDFGGSLTVSNHSEGGAVFRIELALAGET
GQEAAE