Protein Info for SM_b21192 in Sinorhizobium meliloti 1021

Annotation: fructose-1,6-bisphosphate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00167: ketose-bisphosphate aldolase
  • amino acids 1 to 328 (328 residues), 318.5 bits, E=4.2e-99
• TIGR01521: fructose-bisphosphate aldolase, class II, Calvin cycle subtype
  • amino acids 3 to 346 (344 residues), 578.6 bits, E=4.3e-178
• PF01116: F_bP_aldolase
  • amino acids 5 to 328 (324 residues), 319.9 bits, E=8.4e-100

Best Hits

Swiss-Prot: 68% identical to ALF_NOSCO: Fructose-bisphosphate aldolase (fba) from Nostoc commune

KEGG orthology group: K01624, fructose-bisphosphate aldolase, class II [EC: 4.1.2.13] (inferred from 100% identity to smk:Sinme_4846)

MetaCyc: 66% identical to class II fructose-bisphosphate aldolase/sedoheptulose-1,7-bisphosphate aldolase monomer (Synechocystis sp. PCC 6803)
Fructose-bisphosphate aldolase. [EC: 4.1.2.13]; 4.1.2.13 [EC: 4.1.2.13]

Predicted SEED Role

"Fructose-bisphosphate aldolase class II (EC 4.1.2.13)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis (EC 4.1.2.13)

MetaCyc Pathways

• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (22/26 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• Calvin-Benson-Bassham cycle (12/13 steps found)
• gluconeogenesis I (12/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• homolactic fermentation (10/12 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• glycolysis IV (8/10 steps found)
• oxygenic photosynthesis (13/17 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• gluconeogenesis III (9/12 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• sedoheptulose bisphosphate bypass (1/2 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• 1,3-propanediol biosynthesis (engineered) (5/9 steps found)
• sucrose degradation V (sucrose α-glucosidase) (2/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (7/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 4.1.2.13

Use Curated BLAST to search for 4.1.2.13

Sequence Analysis Tools

See Q92V58 at UniProt or InterPro

Protein Sequence (347 amino acids)

>SM_b21192 fructose-1,6-bisphosphate aldolase (Sinorhizobium meliloti 1021)
MALITLRQLLDDAAENDYALPAFNVNNLEYIQAVMRAADATDSPVILQASRGARAYAGDA
FLRHLILGAAEEYPHIPVCLHLDHGDQPSTCISAITNGFTSVMMDGSLEKDGKTVASYEY
NVAVTAEVVKIAHAAGVSVEGELGCLGNLETGAGDKEDGHGFEGKLSREELLTDPEQALD
FVSKTGVDALAVAIGTSHGAYKFTREPDGEILSIETIAKINKRLPNTHLVMHGSSSVPAD
LQELFNAYGGKMKKTWGVPVSEIQKAIPLGVRKVNIDTDLRLAFTGEIRKHHIEHPDNFD
PRNYLKPAIAHMTEVCKERFVAFRAAGQASKIRVLRLPEMAKRYAAA