Protein Info for SM_b21171 in Sinorhizobium meliloti 1021

Annotation: enzyme protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 TIGR02318: phosphonate metabolism protein PhnM" amino acids 7 to 378 (372 residues), 522.7 bits, see alignment E=2.5e-161 PF01979: Amidohydro_1" amino acids 217 to 375 (159 residues), 29.8 bits, see alignment E=3.7e-11 PF07969: Amidohydro_3" amino acids 258 to 378 (121 residues), 38.5 bits, see alignment E=1.1e-13

Best Hits

Swiss-Prot: 48% identical to PHNM_ECOLI: Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (phnM) from Escherichia coli (strain K12)

KEGG orthology group: K06162, PhnM protein (inferred from 100% identity to sme:SM_b21171)

MetaCyc: 100% identical to alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (Sinorhizobium meliloti 1021)
RXN-17958 [EC: 3.6.1.63]

Predicted SEED Role

"Metal-dependent hydrolase involved in phosphonate metabolism" in subsystem Alkylphosphonate utilization

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92V74 at UniProt or InterPro

Protein Sequence (379 amino acids)

>SM_b21171 enzyme protein (Sinorhizobium meliloti 1021)
MPKEQLLTNARIVLEDRILDGSVLIRDGRIADISEGASAAGEDFEGDYLLPGLVELHTDH
LEAHYSPRPGVRWLKIAAIQAHDAQVVTSGITTVFDCLRLGSDEDSGFPKGEMRSMADAL
AQAKQEGRLRADHLIHLRCEVSTADVLEHYEDFRNDPQVRLVSLMDHAPGQRQFQTMDQY
TLYYKTKRGLTDDAFAAFIERQQALSARYATPHRNALAKACAERGITVASHDDATVEHVE
ESIGYGIRLAEFPTSFEAAEASHRAGLSVLMGAPNIVRGKSHSGNIAARDLAKRGVLDVL
SSDYVPFSLIHAPFILADEVESIGLPEAIAMVTATPARTVGLEDRGRIAVGLRADIARVR
RPEGIPVVRTVWREGRRVA