Protein Info for SM_b21022 in Sinorhizobium meliloti 1021

Annotation: autoinducer-2 (AI-2) kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 PF00370: FGGY_N" amino acids 5 to 249 (245 residues), 149.8 bits, see alignment E=1e-47 PF02782: FGGY_C" amino acids 295 to 451 (157 residues), 60.2 bits, see alignment E=2.5e-20

Best Hits

Swiss-Prot: 57% identical to LSRK_KLEP7: Autoinducer-2 kinase (lsrK) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K11216, autoinducer 2 (AI-2) kinase [EC: 2.7.1.-] (inferred from 100% identity to sme:SM_b21022)

MetaCyc: 57% identical to autoinducer-2 kinase (Escherichia coli K-12 substr. MG1655)
RXN0-5461 [EC: 2.7.1.189]

Predicted SEED Role

"Autoinducer 2 (AI-2) kinase LsrK (EC 2.7.1.-)" in subsystem Autoinducer 2 (AI-2) transport and processing (lsrACDBFGE operon) (EC 2.7.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-

Use Curated BLAST to search for 2.7.1.- or 2.7.1.189

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92VV3 at UniProt or InterPro

Protein Sequence (532 amino acids)

>SM_b21022 autoinducer-2 (AI-2) kinase (Sinorhizobium meliloti 1021)
MKTSYMLAVDAGTGSGRAVIFDENGNQIASAQHEWWHRADPRFPGSMDFDVEGNWSLLSR
AIRQAIAESRIAPAEIRAVSATSMREAIVAYDRSGREIWACANVDSRAVSEVRDLQRDFA
DLERALYTESGQTFALGALPRLLWLKRNLPDVYARIHKISMLSDWVLARLSGVIASDPSN
AGTTGLYSLTKRDWMPEALSKAGMRDDIFPESVEPGTVIGRVTEKAASETGIAVGTPVVM
GGGDCQSGAAAVGVVNEGDCAVLGGTFWQQVVNVGPSVSDPSMDLRINPHVVNGLNQAEA
ISFFVGMVMRWFRDSFGAEEVAAAGPGGDAYRLLEEKAAGVPVGAYGIIPVFSDVMHYGN
WYHAAPSLLNLSIDPEKSGKAAIFRALQENAAIVAARNLSAIFKLSGKTPDKIVFAAGAS
KSAHWSQILADATGLPVVTPVVKEATALGCAQRRQSGSAYAATSSRRRRAGCAGTSVSSR
ISTTRLSTTRRASAGHAPTRCSDSSWTRASPRPCGRRQVSEFYRSVNQHSRN