Protein Info for SM_b20899 in Sinorhizobium meliloti 1021

Updated annotation (from data): Inositol 2-dehydrogenase (EC 1.1.1.18)
Rationale: Specifically important for utilizing m-Inositol. Automated validation from mutant phenotype: the predicted function (MYO-INOSITOL-2-DEHYDROGENASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: myo-inositol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 TIGR04380: inositol 2-dehydrogenase" amino acids 3 to 327 (325 residues), 421 bits, see alignment E=1.4e-130 PF01408: GFO_IDH_MocA" amino acids 3 to 120 (118 residues), 84.7 bits, see alignment E=1.6e-27 PF03807: F420_oxidored" amino acids 5 to 85 (81 residues), 26.7 bits, see alignment E=1.4e-09 PF22725: GFO_IDH_MocA_C3" amino acids 128 to 248 (121 residues), 109.1 bits, see alignment E=3e-35 PF02894: GFO_IDH_MocA_C" amino acids 132 to 328 (197 residues), 102.7 bits, see alignment E=5.2e-33

Best Hits

Swiss-Prot: 100% identical to MI2D_RHIME: Inositol 2-dehydrogenase (idhA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00010, myo-inositol 2-dehydrogenase [EC: 1.1.1.18] (inferred from 99% identity to smk:Sinme_4524)

Predicted SEED Role

"Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18)" in subsystem Inositol catabolism (EC 1.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.18

Use Curated BLAST to search for 1.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See O68965 at UniProt or InterPro

Protein Sequence (330 amino acids)

>SM_b20899 Inositol 2-dehydrogenase (EC 1.1.1.18) (Sinorhizobium meliloti 1021)
MTVRFGLLGAGRIGKVHAKAVSGNADARLVAVADAFPAAAEAIAGAYGCEVRTIDAIEAA
ADIDAVVICTPTDTHADLIERFARAGKAIFCEKPIDLDAERVRACLKVVSDTKAKLMVGF
NRRFDPHFMAVRKAIDDGRIGEVEMVTITSRDPSAPPVDYIKRSGGIFRDMTIHDFDMAR
FLLGEEPVSVTATAAVLIDKAIGDAGDYDSVSVILQTASGKQAIISNSRRATYGYDQRIE
VHGSKGAVAAENQRPVSIEIATGDGYTRPPLHDFFMTRYTEAYANEIESFIAAIEKGAEI
APSGNDGLAALALADAAVRSVAEKRQISIA