Protein Info for SM_b20869 in Sinorhizobium meliloti 1021

Annotation: two-component response regulator protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 PF00072: Response_reg" amino acids 26 to 136 (111 residues), 83.7 bits, see alignment E=1.1e-27 PF00196: GerE" amino acids 162 to 217 (56 residues), 65.2 bits, see alignment E=3.2e-22

Best Hits

Swiss-Prot: 49% identical to NODW_BRADU: Nodulation protein W (nodW) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: None (inferred from 99% identity to smk:Sinme_4555)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92UG8 at UniProt or InterPro

Protein Sequence (233 amino acids)

>SM_b20869 two-component response regulator protein (Sinorhizobium meliloti 1021)
MRLSQSAGTSAPLLSQRKKSSAESIVVVVDDDAAVREGLSELILSAGFQPVSFASTQELL
NAVDILDGPGCLILDVHMPGESGLDLQHHLAERGIGKPIIFLTGRGDIPMTVQAMKDGAV
DFLTKPVSDRTLLDAVIAAIALDQTQRAEAIVVKRNVERLKTLTRREREVLREVVTRGRL
NKQIAFDLGISEVTVKLHRANVMRKMEVRSIGELIRVWETLPPTVRERAEAGF