Protein Info for SM_b20849 in Sinorhizobium meliloti 1021

Annotation: guanine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02967: guanine deaminase
  • amino acids 27 to 428 (402 residues), 587.6 bits, E=6.2e-181
• PF01979: Amidohydro_1
  • amino acids 68 to 430 (363 residues), 175.6 bits, E=1.7e-55
• PF07969: Amidohydro_3
  • amino acids 256 to 430 (175 residues), 45 bits, E=1.1e-15

Best Hits

Swiss-Prot: 48% identical to GUAD_ECOLI: Guanine deaminase (guaD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4576)

MetaCyc: 48% identical to guanine deaminase (Escherichia coli K-12 substr. MG1655)
Guanine deaminase. [EC: 3.5.4.3]

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3)" in subsystem Purine Utilization or Purine conversions (EC 3.5.4.3)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3

Use Curated BLAST to search for 3.5.4.3

Sequence Analysis Tools

See Q92UI8 at UniProt or InterPro

Protein Sequence (438 amino acids)

>SM_b20849 guanine deaminase (Sinorhizobium meliloti 1021)
MTSTLIRGRLLSFHREPQGIDDSAAYAYESDGALLVENGVIKASGSYAAVKAAAPEEVPE
IDHRPHLIVPGLIDTHLHFPQMQVMASYAADLLEWLNTYTFPEECRFVETAHAERIARHF
FDEMIRHGTTTATAYCSVHKTSADAFFAESLKRGMCMVAGKVMMDRNAPQGLLDTPEMSY
DETRAVIADWHGKGRNHVAITPRFAITSTPEQMEVAKSLVGEFPDLHVQTHLSENRDEIA
YTSELYPEATDYTDVYARYGLLGPKSLFGHCIHLSEREADAMSETGSVAVFCPTSNLFLG
SGLFPLRGLKRRQKPVRVSVASDIGGGTSYSMLKTLDEAYKILQLQGERLNPFDSFYMMT
RGNAEALSLADRIGTLEPGTDADIAVLDMAATPAMALRAEVVNSLADELFLLQTMGDDRA
VVETYVAGKPCKSTLGAT