Protein Info for SM_b20756 in Sinorhizobium meliloti 1021

Annotation: propionyl-CoA carboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 4 to 113 (110 residues), 154.7 bits, E=4.6e-49
• PF02786: CPSase_L_D2
  • amino acids 118 to 325 (208 residues), 274.4 bits, E=2.4e-85
• PF08443: RimK
  • amino acids 118 to 317 (200 residues), 21.3 bits, E=7.2e-08
• PF02222: ATP-grasp
  • amino acids 140 to 295 (156 residues), 28.9 bits, E=3.4e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 145 to 294 (150 residues), 36.1 bits, E=2e-12
• PF02785: Biotin_carb_C
  • amino acids 339 to 451 (113 residues), 114.5 bits, E=1.1e-36
• PF18140: PCC_BT
  • amino acids 471 to 595 (125 residues), 117.4 bits, E=1.9e-37
• PF00364: Biotin_lipoyl
  • amino acids 606 to 668 (63 residues), 54.4 bits, E=3.7e-18

Best Hits

KEGG orthology group: K01965, propionyl-CoA carboxylase alpha chain [EC: 6.4.1.3] (inferred from 100% identity to sme:SM_b20756)

MetaCyc: 69% identical to propionyl-CoA carboxylase alpha subunit (Cereibacter sphaeroides)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module (EC 6.4.1.3)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• mycolate biosynthesis (21/205 steps found)
• superpathway of mycolate biosynthesis (22/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Sequence Analysis Tools

See Q92TQ2 at UniProt or InterPro

Protein Sequence (670 amino acids)

>SM_b20756 propionyl-CoA carboxylase subunit alpha (Sinorhizobium meliloti 1021)
MGHMFKKILIANRGEIACRVIRTTKALGIPTVAVYSDADRDAMHVRMADEAVHIGPSPSS
QSYIVIENILAAIRRTGADAVHPGYGFLSENAAFAEALEKDGVTFIGPPVRAIEAMGDKI
TSKKLAAEAGVFTVPGHMGLIEDADEAARIAAEIGFPVMIKASAGGGGKGMRIAWNEREA
REGFQSSRNEAKSSFGDDRIFIEKFVTEPRHIEIQVLGDKHGNILYLGERECSIQRRNQK
VIEEAPSPFLDEKTRRAMGEQAVALAKAVGYHSAGTVEFIVDAGRNFYFLEMNTRLQVEH
PVTELVTGLDLVEQMIRVAAGAKLAFAQKDVKLDGWAIESRLYAEDPYRTFLPSIGRLTR
YRPPEEGTQADGTVIRNDTGVFEGGEISMYYDPMIAKLCTWGPDRLTAVRAMADALDAFE
VEGIGHNLPFLAAVMQQERFHEGRLTTAYIAEEFAGGFHGVALDDASARKLAAVAATVNQ
TLQERASRISGTIGNHRRVVGHEWVTSLDGHEIQVTCEVSADGTYVRFADGTSVSVATDW
APGRTRAAFNIDNQPMSVKVELAGPGIRLRWRGIDVVARVRSPRIAELARLMPKKLPPDT
SKMLLCPMPGVVTSITVKAGETVEAGQAIAVVEAMKMENILRAEKRAIVKRVAIEAGASL
AVDELIMEFE