Protein Info for SM_b20751 in Sinorhizobium meliloti 1021

Annotation: 3-hydroxyisobutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF03807: F420_oxidored" amino acids 3 to 69 (67 residues), 34.4 bits, see alignment E=5.7e-12 PF03446: NAD_binding_2" amino acids 3 to 159 (157 residues), 170 bits, see alignment E=9e-54 TIGR01692: 3-hydroxyisobutyrate dehydrogenase" amino acids 6 to 290 (285 residues), 417.4 bits, see alignment E=1.5e-129 PF14833: NAD_binding_11" amino acids 162 to 289 (128 residues), 122.4 bits, see alignment E=2.5e-39

Best Hits

Swiss-Prot: 57% identical to MMSB_MYCTO: Probable 3-hydroxyisobutyrate dehydrogenase (mmsB) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: None (inferred from 100% identity to smk:Sinme_4291)

MetaCyc: 50% identical to 3-hydroxyisobutyrate dehydrogenase subunit (Pseudomonas putida)
3-hydroxyisobutyrate dehydrogenase. [EC: 1.1.1.31]

Predicted SEED Role

"3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.1.1.31)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.31

Use Curated BLAST to search for 1.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TQ7 at UniProt or InterPro

Protein Sequence (298 amino acids)

>SM_b20751 3-hydroxyisobutyrate dehydrogenase (Sinorhizobium meliloti 1021)
MTKIAFIGLGNMGGPMAANLVKAGHAVTGFDLSEASRNAAAKTGVSVAGSIAQAVREMEC
VITMLPASAHVLYVWDELLGFVDPGTLLIDSSTIDVESARKVHGFAEKAGCPSLDAPVSG
GTAGAAAATLTFMVGGSEGAFSRGKHLLEAMGKKIVHCGDAGAGQAAKICNNMILGVSMA
AVCEAFVLAERLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANGGYKPGFAASL
MLKDLKLSQQAASASGAATPMGAQATQLYSLFEKLGHGAEDFSALIHLLRGNEEAKEG