Protein Info for SM_b20749 in Sinorhizobium meliloti 1021

Annotation: D-mannonate oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details PF01232: Mannitol_dh" amino acids 28 to 187 (160 residues), 142.6 bits, see alignment E=1.2e-45 PF08125: Mannitol_dh_C" amino acids 215 to 458 (244 residues), 276.4 bits, see alignment E=2.2e-86

Best Hits

Swiss-Prot: 52% identical to UXUB_ECOLI: D-mannonate oxidoreductase (uxuB) from Escherichia coli (strain K12)

KEGG orthology group: K00040, fructuronate reductase [EC: 1.1.1.57] (inferred from 100% identity to smk:Sinme_4293)

MetaCyc: 52% identical to D-mannonate oxidoreductase (Escherichia coli K-12 substr. MG1655)
Fructuronate reductase. [EC: 1.1.1.57]

Predicted SEED Role

"D-mannonate oxidoreductase (EC 1.1.1.57)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.57)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.57

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TQ9 at UniProt or InterPro

Protein Sequence (487 amino acids)

>SM_b20749 D-mannonate oxidoreductase (Sinorhizobium meliloti 1021)
MRLSRKTIDRLPRTVKRPHYDLGTVTVGIVHLGIGAFHRAHQAVYTDGLLSEDPSWGICG
VSLRSPETRDALHPQDGLYTLAVQDGEGSELSVVGSVVELLCAPDDPEAVLRRMADPGTR
IVSLTITEKGYCHNPATGTLDEGHPDIVHDLANPARPRSAIGFIVEAISRRVSAGIAPFT
LLSCDNLPGNGHVLKRIVTQFAEARDPALAAVVRNVASPSTMVDRIVPATTDSDRSAVAS
AMGLEDAWPIMTEPFRQWVIEEDFPLGRPAWEKAGALFVQDVSAFEFMKLRLLNGSHSTL
AYLGYLAGAETVADAMALAGMEALVEGLMRHEVSPTLPELPGFDLPAYRAELLQRFRNPA
LRHRTWQIAMDGSQKLPQRLLGSIRDRLQAGAGYDRLALGVAAWMRYARGLDEAGRPIDV
RDPHAARIAGLARGIDEPDRIVDAFLTMTDVFGTDLPASAPFRAALIKALDGLLRIGAAA
TLRRFRR