Protein Info for SM_b20709 in Sinorhizobium meliloti 1021

Annotation: 3-methyladenine DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 transmembrane" amino acids 71 to 96 (26 residues), see Phobius details TIGR00567: DNA-3-methyladenine glycosylase" amino acids 8 to 182 (175 residues), 152.5 bits, see alignment E=4.9e-49 PF02245: Pur_DNA_glyco" amino acids 9 to 178 (170 residues), 189.7 bits, see alignment E=1.5e-60

Best Hits

Swiss-Prot: 100% identical to 3MGH_RHIME: Putative 3-methyladenine DNA glycosylase (RB1416) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03652, DNA-3-methyladenine glycosylase [EC: 3.2.2.21] (inferred from 100% identity to smk:Sinme_4320)

Predicted SEED Role

"DNA-3-methyladenine glycosylase II (EC 3.2.2.21)" in subsystem DNA Repair Base Excision (EC 3.2.2.21)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.21

Use Curated BLAST to search for 3.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TT1 at UniProt or InterPro

Protein Sequence (185 amino acids)

>SM_b20709 3-methyladenine DNA glycosylase (Sinorhizobium meliloti 1021)
MHRLTSDVDFFARSAVQVAADLIGADFTVSGVGGTIVETEAYLPDDAASHSFAGTTARNR
AMFGPPAHAYIYLSYGLHWCLNFVCLPGSAVLIRAIEPRWGIDTMRARRGVREERLLCSG
PGRVGQALAISRELDGLPLGEDPFRLTLPSTKPPLAAGIRVGITKAVEQPWRFGLAGSSF
VSRKF