Protein Info for SM_b20685 in Sinorhizobium meliloti 1021

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 818 TIGR02777: DNA ligase D, 3'-phosphoesterase domain" amino acids 6 to 160 (155 residues), 233 bits, see alignment E=2.6e-73 PF13298: LigD_N" amino acids 35 to 140 (106 residues), 146.6 bits, see alignment E=5.6e-47 PF01068: DNA_ligase_A_M" amino acids 232 to 408 (177 residues), 94.8 bits, see alignment E=1.2e-30 TIGR02779: DNA ligase D, ligase domain" amino acids 233 to 520 (288 residues), 311.2 bits, see alignment E=1.4e-96 TIGR02776: DNA ligase D" amino acids 238 to 810 (573 residues), 736.4 bits, see alignment E=6e-225 PF04679: DNA_ligase_A_C" amino acids 427 to 513 (87 residues), 67.4 bits, see alignment E=2.5e-22 TIGR02778: DNA ligase D, polymerase domain" amino acids 536 to 781 (246 residues), 323.5 bits, see alignment E=2e-100 PF21686: LigD_Prim-Pol" amino acids 552 to 805 (254 residues), 282.7 bits, see alignment E=6e-88

Best Hits

KEGG orthology group: None (inferred from 100% identity to sme:SM_b20685)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q92TV3 at UniProt or InterPro

Protein Sequence (818 amino acids)

>SM_b20685 hypothetical protein (Sinorhizobium meliloti 1021)
MATPKLEAYRKKRDFSKTPEPVGNLTAGGNRFVVHKHHATADHYDLRLEVDGVLKSWAVP
KGPSLNPADKRLAVETEDHPLDYIDFEGVIPEGEYGGGPMIVWDKGVWAPMGDVDDDLRK
GAFKFRLAGEKLNGGWMLARLKRRPGEEDQRNWLLFKERDPAADTSIDILAARPESVKSG
RRIEELVEKPKAPPKPVKLNPGALAGAVKAAQPERIEPQLATQTILPPEAEKDSKKPERW
LHEIKFDGYRTMAHVADGKVRLVTRGGLDWTKRYGDLPEVFRRLPCRDVIIDGEIVVLDE
TGVSRFALLQDALSTGAGNSLVFYAFDLLHLNGWNLFDVPLEKRKALLKQLLAGQVSSRS
AIQFSDHVLGEGRALYDRASEMGLEGIVSKRISAPYRSGRSKTWTKTKALKAEDFVIVGY
TVSEAAEGIASLALGEWADGELEYRGKVGTGFDAQMLKELLARLEPLRAGAAKLEGAPRE
IIWVRPLLRAHIHYGNRTTDNVLRHAVFKGLRDVELSTPVSMSRKRLISDADLAGISITN
PTRRLFGKSGPTKLDLAVYYAMIGDFMLPHILGRPVSLVRCPTGRTQDCFFQRHPFTGMP
PSVATFQATNSEGEAKTYLSVEDARGYLALAQFGVVEFHNWGTTRKLLGKPDRVVFDLDP
GEGIAWREVVEAAIHIKAELEALALVPFVKTSGGRGVHVVVPVVPKLDWKKFHQATSALA
TRLAATAPATFTTTMGKDNRIRRIFIDFHRNARSHTWAAPYSLRARTNLPASTPLSWADL
ETIDAPADLNYSSLPGLLATSGDPWADIDDFARDLPIL